Aptamer-based microchip electrophoresis assays for amplification detection of carcinoembryonic antigen

Clinica Chimica Acta; International Journal of Clinical Chemistry
Li PanYi-Ming Liu

Abstract

Carcinoembryonic antigen (CEA) as one of the most widely used tumor markers is used in the clinical diagnosis of colorectal, pancreatic, gastric, and cervical carcinomas. We developed an aptamer-based microchip electrophoresis assay technique for assaying CEA in human serum for cancer diagnosis. The magnetic beads (MBs) are employed as carriers of double strand DNA that is formed by an aptamer of the target and a complementary DNA of the aptamer. After the aptamer in the MB-dsDNA conjugate binds with the target, the complementary DNA was released from the MB-dsDNA conjugate. The released complementary DNA hybridizes with a fluorescein amidite (FAM) labeled DNA, and forms a DNA duplex, which triggers the selective cleavage of FAM labeled DNA by nicking endonuclease Nb.BbvCI, and generating a FAM labeled DNA segment. The released complementary DNA hybridizes with another FAM labeled DNA, resulting in a continuous cleavage of FAM labeled DNA, and the generation of large numbers of FAM labeled DNA segments. In MCE laser induced fluorescence detection (LIF), the FAM labeled DNA segment is separated and detected. The linear range for CEA was 130 pg/ml-8.0 ng/ml with a correlation coefficient of 0.9916 and a detection limit of 68 pg/m...Continue Reading

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Citations

Jan 1, 2019·Journal of Drug Targeting·Ge GaoJuewen Liu
May 23, 2019·The Analyst·Chuanpin ChenTingting Hong
Jan 25, 2020·Electrophoresis·Hana NevídalováZdeněk Glatz
Feb 26, 2020·Micromachines·Niazul I Khan, Edward Song
Nov 15, 2018·Micromachines·Donny Nugraha MazaafriantoManabu Tokeshi

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