Aptamer-based surface-enhanced Raman scattering-microfluidic sensor for sensitive and selective polychlorinated biphenyls detection

Analytical Chemistry
Cuicui FuWeiqing Xu

Abstract

A surface-enhanced Raman scattering (SERS) measurement of 3,3',4,4'-tetrachlorobiphenyl (PCB77) with aptamer capturing in a microfluidic device was demonstrated. To construct the microfluidic chip, an ordered Ag nanocrown array was fabricated over a patterned polydimethylsiloxane (PDMS) that was achieved by replicating an anodic aluminum oxide (AAO) template. The patterned PDMS sheet was covered with another PDMS sheet having two input channel grooves to form a close chip. The Ag nanocrown array was used for the SERS enhancement area and the detection zone. PCB 77 aptamers were injected into one channel and the other allows for analytes (PCBs). The mercapto aptamers captured the targets in the mixed zone and were immobilized to the SERS detection zone via S-Ag bonds so as to further improve both the SERS sensitivity and selectivity of PCB77. Such an aptamer-based microfluidic chip realized a rapid SERS detection. The lowest detectable concentration of 1.0 × 10(-8) M was achieved for PCB77. This work demonstrates that the aptamer-modified SERS microfluidic sensor can be utilized for selective detections of organic pollutants in the environment.

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Citations

Jul 6, 2016·Frontiers in Chemistry·Franziska Pfeiffer, Günter Mayer
May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner
Jun 12, 2019·Journal of Healthcare Engineering·Elisa ScatenaCostantino Del Gaudio
Aug 1, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zahra Rezaei, Matin Mahmoudifard
Nov 24, 2020·Analytical Methods : Advancing Methods and Applications·Shiqiang WangMingwei Tian
Nov 6, 2018·Biosensors & Bioelectronics·Shan LiangMin Zhang
Jan 29, 2021·Nanomaterials·Rebeca MoldovanRadu Oprean
Nov 2, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sijia LiuXianming Kong
Jul 30, 2020·Journal of Hazardous Materials·Asma VerdianEbrahim Fooladi
Jul 18, 2018·Environmental Science & Technology·Spurti U Akki, Charles J Werth
Aug 27, 2021·Advanced Optical Materials·Xiaolei PengYuebing Zheng
Aug 23, 2018·ACS Applied Materials & Interfaces·Victoria M SzlagChristy L Haynes
Nov 21, 2021·Journal of Agricultural and Food Chemistry·Mengmeng YanZilei Chen

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