Aptamer Display on Diverse DNA Polyhedron Supports

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Simon Chi-Chin ShiuJulian A Tanner

Abstract

DNA aptamers are important tools for molecular recognition, particularly for a new generation of tools for biomedicine based on nucleic acid nanostructures. Here, we investigated the relative abilities of different shapes and sizes of DNA polyhedra to display an aptamer which binds to the malaria biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH). The aptamer was shown to perform an Aptamer-Tethered Enzyme Capture (APTEC) assay with the hypothesis that the display of the aptamer above the surface through the use of a polyhedron may lead to better sensitivity than use of the aptamer alone. We compared different numbers of points of contact, different shapes, including tetrahedron, square, and pentagon-based pyramids, as well as prisms. We also investigated the optimal height of display of the structure. Our results demonstrated that the display of an aptamer on an optimized nanostructure improved sensitivity up to 6-fold relative to the aptamer alone in the APTEC assay. Other important factors included multiple basal points of contact with the surface, a tetrahedron proved superior to the more complex shaped structures, and height above the surface only made minor differences to efficacy. The display of an aptamer on ...Continue Reading

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Citations

Jul 15, 2018·Chembiochem : a European Journal of Chemical Biology·Simon Chi-Chin ShiuJulian A Tanner
May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner
Nov 30, 2018·Genes·Juan David Ospina-VillaLaurence A Marchat
Jun 23, 2021·Chemical Reviews·Abhichart KrissanaprasitThomas H LaBean
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li
Oct 9, 2021·Signal Transduction and Targeted Therapy·Wenjuan MaYunfeng Lin

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Methods Mentioned

BETA
Enzyme Capture
electrophoresis
PCR

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