Aptamer functionalized curcumin-loaded human serum albumin (HSA) nanoparticles for targeted delivery to HER-2 positive breast cancer cells

International Journal of Biological Macromolecules
Tayebeh SalehSeyed Abbas Shojaosadati

Abstract

In this study, an HER2 aptamer-decorated curcumin-loaded human serum albumin nanoparticle (Apt-HSA/CCM NP) was developed and characterized as a new anticancer formulation for targeted delivery to human epithelial growth factor receptor 2 (HER2) overexpressing breast cancer cells. Conjugation of HER2 Apt to the surface of HSA NPs was confirmed by gel electrophoresis and FTIR analysis. The obtained NPs have the hydrodynamic diameter of 281.1 ± 11.1 nm and zeta potential of -33.3 ± 2.5 mV. The data demonstrated that encapsulation of curcumin in HSA NPs by desolvation method has increased water solubility by 400 folds. Fluorescent microscopy image demonstrated remarkable cytoplasmic uptake of Apt-HSA/CCM NPs in HER2-overexpressing SK-BR-3 cells compared to unconjugated counterparts. Cytotoxicity experiments demonstrated no significant difference between cytotoxic effect of free curcumin and non-targeted HSA/CCM NPs in both HER2 positive and HER2 negative cell lines. However, the toxicity of Apt-HSA/CCM NPs was significantly higher and cell viability reached 36% after 72 h in SK-BR3 cell line. These results suggest that this targeted delivery system has the potential to be considered as a promising candidate for the treatment of HER...Continue Reading

Citations

Aug 1, 2019·Current Drug Targets·Jobin JoseBijo Mathew
Aug 1, 2019·Current Pharmaceutical Biotechnology·Hasanain Gomhor J AlqaraghuliRonak Rafipour
Feb 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mahshid Moballegh NaseryGautam Sethi
Jul 3, 2020·Pharmaceutics·Seyoung HongHee Ho Park
Sep 8, 2020·Drug Development and Industrial Pharmacy·Hossein AbolhassaniSeyed Abbas Shojaosadati
Oct 4, 2020·IET Nanobiotechnology·Hasnat Tariq, Syed Ali Imran Bokhari
Apr 23, 2020·International Journal of Molecular Sciences·Zhaoying Fu, Jim Xiang
Apr 30, 2020·Mini Reviews in Medicinal Chemistry·Ayushi SethiyaShikha Agarwal
Dec 4, 2020·International Journal of Molecular Sciences·Zhaoying Fu, Jim Xiang
Sep 2, 2020·Advanced Drug Delivery Reviews·Anastasia VarankoAshutosh Chilkoti
Feb 2, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhi ZhuDusan Losic
Feb 15, 2020·International Journal of Biological Macromolecules·Asiye AkbarianZuhair Mohammad Hassan
Feb 5, 2021·Biomedical Materials·Thais S CorreaMariana Campos-da-Paz
Sep 30, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuangshuang ZhangHui Li
Jan 16, 2022·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yunjian LiWeisan Pan

❮ Previous
Next ❯

Related Concepts