Aptamer-mediated Plasmodium-specific diagnosis of malaria

Biochimie
Yee-Wai CheungJulian A Tanner

Abstract

There is a critical need for better malaria rapid diagnostic tests to discriminate Plasmodium falciparum and Plasmodium vivax infection given the recent observation of HRP2 deletions in P. falciparum parasites. We previously identified a DNA aptamer, 2008s, that targets P. falciparum lactate dehydrogenase (PfLDH) and developed a sensitive aptamer-tethered enzyme capture (APTEC) assay. Here, we characterise two different LDH-binding DNA aptamers in their species-specific activities, then integrate within biochemical diagnostic assays and test in clinical samples. An enzyme-linked oligonucleotide assay demonstrated that aptamer pL1 bound with high affinity to both PfLDH and P. vivax lactate dehydrogenase (PvLDH), whereas aptamer 2008s was specific to PfLDH. An aptamer-tethered enzyme capture (APTEC) assay confirmed the specificity of 2008s in binding and capturing the enzyme activity of PfLDH which could be observed colorimetrically. In malaria patient samples, the 2008s APTEC assay was specific for P. falciparum blood samples and could discriminate against P. vivax blood samples. An aptamer for specific detection of falciparum malaria holds promise as a new strategy for species-specific malaria diagnosis rather than the conventi...Continue Reading

Citations

Mar 10, 2018·International Journal of Molecular Sciences·Wesley O TuckerJulian A Tanner
Jul 15, 2018·Chembiochem : a European Journal of Chemical Biology·Simon Chi-Chin ShiuJulian A Tanner
May 29, 2020·Biomédica : revista del Instituto Nacional de Salud·Juan David Ospina
Oct 6, 2019·Scientific Reports·Adar HacohenIdo Bachelet
Nov 30, 2018·Genes·Juan David Ospina-VillaLaurence A Marchat
May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner
Jun 27, 2020·Applied Microbiology and Biotechnology·Fernanda Raquel Wust SchmitzDachamir Hotza
Jul 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yee-Wai CheungJulian A Tanner
Jul 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simon Chi-Chin ShiuJulian A Tanner
Apr 10, 2019·PloS One·Diego F JosephPohl Milón
Dec 3, 2020·Nature Communications·Antonio MinopoliRaffaele Velotta
Jul 6, 2021·Frontiers in Cellular and Infection Microbiology·Alba Marina GimenezDaniel Youssef Bargieri
Jan 29, 2022·ACS Applied Materials & Interfaces·Antonio MinopoliRaffaele Velotta

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