Aptamer selection for the inhibition of cell adhesion with fibronectin as target

Bioorganic & Medicinal Chemistry Letters
Atsushi OgawaYasuhiro Aoyama

Abstract

An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.

References

Dec 1, 1992·Scientific American·G F Joyce
Aug 30, 1990·Nature·A D Ellington, J W Szostak
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D JellinekN Janjić
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·D O'ConnellA Varki
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·W Xu, A D Ellington
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·M BlindM Famulok
Jun 29, 2000·Annual Review of Biochemistry·D S Wilson, J W Szostak
Sep 20, 2000·Accounts of Chemical Research·M FamulokM Blind
Feb 17, 2001·Biochemical and Biophysical Research Communications·S JeongJ Yu
Sep 27, 2001·Journal of Cellular Physiology·E H Danen, K M Yamada
Oct 26, 2001·Bioorganic & Medicinal Chemistry Letters·E FukusakiA Kobayashi
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Tatsuo OhmichiEric T Kool
Apr 1, 1997·Chemical Reviews·Ronald R. Breaker
Feb 22, 2002·European Journal of Biochemistry·Michiko KimotoShigeyuki Yokoyama
Apr 25, 2002·Journal of the American Chemical Society·Richard P Fahlman, Dipankar Sen
Jun 13, 2002·Journal of the American Chemical Society·Akinori KuzuyaMakoto Komiyama
Oct 17, 2002·Journal of the American Chemical Society·Phillip Ordoukhanian, Gerald F Joyce
Jan 9, 2003·Journal of the American Chemical Society·Shirley H J MeiYingfu Li
Jul 3, 2003·Chemistry & Biology·Markus SchüttLuis Moroder
Jul 3, 2003·Chemistry & Biology·Allen R BuskirkDavid R Liu
Oct 3, 2003·Chembiochem : a European Journal of Chemical Biology·Martina Rimmele
Feb 26, 2004·Bioorganic & Medicinal Chemistry·Mohammad Mehedi MasudHiroaki Sawai

❮ Previous
Next ❯

Citations

Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Feb 9, 2012·The Analyst·Citartan MarimuthuSubash C B Gopinath
Nov 23, 2006·BMC Bioinformatics·Venkata ThodimaYouping Deng
Jan 1, 2010·International Journal of Molecular Sciences·Maureen McKeagueMaria C Derosa
Dec 18, 2012·International Journal of Molecular Sciences·Elsa Rouah-MartinJohan Robbens
Nov 29, 2014·Expert Opinion on Drug Delivery·Jie LiaoHuixia Liu
Mar 27, 2010·Advanced Drug Delivery Reviews·Jung Heon LeeYi Lu
Jan 16, 2007·Computational Biology and Chemistry·Howard A Levine, Marit Nilsen-Hamilton
Oct 10, 2014·PloS One·András SzilágyiEörs Szathmáry
Jun 7, 2021·Analytical and Bioanalytical Chemistry·Ramón Lorenzo-GómezMaría Jesús Lobo-Castañón

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Annual Review of Medicine
Shahid M NimjeeB A Sullenger
Analytical Chemistry
Giridharan GokulranganGeorge S Wilson
Nucleic Acids Research. Supplement
Hiroshi SaitohHiroaki Sawai
Nature Reviews. Drug Discovery
Eugene W M NgAnthony P Adamis
© 2022 Meta ULC. All rights reserved