Aptamer sensor for cocaine using minor groove binder based energy transfer

Analytica Chimica Acta
Jinwen ZhouNicolas H Voelcker

Abstract

We report on an optical aptamer sensor for cocaine detection. The cocaine sensitive fluorescein isothiocyanate (FITC)-labeled aptamer underwent a conformational change from a partial single-stranded DNA with a short hairpin to a double-stranded T-junction in the presence of the target. The DNA minor groove binder Hoechst 33342 selectively bound to the double-stranded T-junction, bringing the dye within the Förster radius of FITC, and therefore initiating minor groove binder based energy transfer (MBET), and reporting on the presence of cocaine. The sensor showed a detection limit of 0.2 μM. The sensor was also implemented on a carboxy-functionalized polydimethylsiloxane (PDMS) surface by covalently immobilizing DNA aptamers. The ability of surface-bound cocaine detection is crucial for the development of microfluidic sensors.

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Citations

Feb 13, 2016·Analytical Chemistry·Miguel A D NevesMichael Thompson
Jan 7, 2015·Biosensors & Bioelectronics·Ahad MokhtarzadehMohammad Ramezani
Jun 7, 2015·Drug and Alcohol Dependence·Nicolaos ChristodoulidesJohn T McDevitt
May 30, 2015·Biosensors & Bioelectronics·Miguel A D NevesMichael Thompson
Jan 1, 2015·Analytical Chemistry·Katharina UrmannEster Segal
Dec 27, 2017·Sensors·Mariia AndrianovaAlexander Kuznetsov
Jun 7, 2018·ChemistryOpen·Eva GarridoFélix Sancenón
May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner

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