Aptamer surface functionalization of microfluidic devices using dendrimers as multi-handled templates and its application in sensitive detections of foodborne pathogenic bacteria

Analytica Chimica Acta
Xingkai HaoXudong Cao

Abstract

A microfluidic system that incorporates both dendrimers and aptamers to detect E. coli O157:H7 is developed. To achieve this, generation 7-polyamidoamine dendrimers were immobilized onto the detection surfaces of PDMS microfluidic channels; subsequently aptamers against E. coli O157:H7 were conjugated onto the microchannel surfaces via the immobilized dendrimers as templates. Surface modifications were characterized by FTIR, XPS, water contact angles, fluorescence microscopy and AFM to confirm the success of each surface modification steps. The efficacy of this simple microchannel in detection was investigated using E. coli O157:H7 spiked samples. Our results showed that this interesting approach significantly increased the amount of aptamers available on the microfluidic channel surfaces to capture E. coli O157:H7 cells to allow sensitive detection, which in turn resulted in detections of E. coli O157:H7 cells at a low limit of detection of 102 cells mL-1. The results also demonstrated that in comparison with the generation 4-polyamidoamine dendrimers (G4) modified microchannels, those modified with G7 showed enhanced detection signals, improved target capturing efficiencies, and at higher throughput. This simple whole cell de...Continue Reading

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Citations

Dec 8, 2019·Sensors·Muqsit Pirzada, Zeynep Altintas
Feb 29, 2020·International Journal of Nanomedicine·Baozhang Guan, Xingwang Zhang
Mar 9, 2021·Frontiers in Microbiology·Mariam Saad, Sebastien P Faucher
May 17, 2021·Food Research International·Xiaoying HanYing Mu

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