Aptamer–biotin–streptavidin–C1q complexes can trigger the classical complement pathway to kill cancer cells.

In Vitro Cellular & Developmental Biology. Animal
John Gordon Bruno

Abstract

Nucleic acid aptamers are regarded as rivals for antibodies and as such are being investigated for their therapeutic potential. In the present work, it is shown that two different high-affinity DNA aptamers developed previously by Ferreira et al. against MUC1 antigen (designated MUC1-5TR-1 and MUC1-S1.3/S2.2) on MCF7 breast cancer cells can be linked to the first component of complement (C1q) via a biotin–streptavidin system and induce significant killing of MCF7 cells in vitro. Cell viability was assessed by Trypan blue uptake and absorbance at 590 nm of stained cells following buffer washes and lysis in 1% SDS. While the killing effect is demonstrable versus various controls, dependent on aptamer dose, and reproducible, it appears to kill maximally about half of treated MCF7 cells. Possible reasons for the marginal killing effect include antigenic shedding in vitro and membrane-bound complement regulatory proteins (mCRPs) on the cell surface such as CD46, CD55, and CD59 which act to inhibit complement-mediated lysis of cells. Future in vitro research could benefit from application of mCRP-specific aptamers in combination with anti-MUC1 aptamers to overcome surface protective mechanisms while attacking the plasma membrane of M...Continue Reading

References

Jan 1, 1994·Springer Seminars in Immunopathology·M C Moffitt, M M Frank
Sep 15, 1998·The American Journal of Pathology·J Hakulinen, S Meri
Jul 27, 1999·International Journal of Cancer. Journal International Du Cancer·A K ChanP S Goedegebuure
Jun 3, 2005·Cancer Research·Jinguo ChenLurong Zhang
Feb 18, 2006·Cancer Immunology, Immunotherapy : CII·Antje DanielczykSteffen Goletz
Jun 17, 2006·Cancer Research·Ted C ChuMatthew Levy
Oct 13, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·C S M FerreiraS Missailidis
Dec 19, 2006·Molecular Cancer Therapeutics·Christopher R Ireson, Lloyd R Kelland
Mar 4, 2008·In Vitro Cellular & Developmental Biology. Animal·John G BrunoBlythe King
May 15, 2008·Expert Opinion on Biological Therapy·Siao-Yi Wang, George Weiner
Sep 30, 2008·International Journal of Cancer. Journal International Du Cancer·Daniel B RubinsteinDaniel H Wreschner
Dec 24, 2008·Nucleic Acids Research·Cátia S M FerreiraJean Gariépy
Mar 4, 2009·Chembiochem : a European Journal of Chemical Biology·Yu-Fen HuangWeihong Tan
Apr 21, 2009·Methods in Molecular Biology·Laura CerchiaVittorio de Franciscis

❮ Previous
Next ❯

Citations

Mar 8, 2013·Future Microbiology·Veli Cengiz ÖzalpHüseyin Avni Öktem
Sep 6, 2012·Journal of Cellular Biochemistry·KuanCan LiuXiaoPeng Lan
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·John G Bruno
Aug 19, 2014·ChemMedChem·Sven KruspeUlrich Hahn
Aug 2, 2017·Medicinal Research Reviews·Maryam Sadat NabaviniaKhalil Abnous
Jun 22, 2018·Oncotarget·Ricardo L PereiraHenning Ulrich
Dec 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mahin ShahdordizadehSeyed Mohammad Taghdisi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.