Aptamers vs. antibodies as capture probes in optical porous silicon biosensors

The Analyst
Sofia Arshavsky-GrahamEster Segal

Abstract

Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regene...Continue Reading

References

Jan 1, 1997·Biosensors & Bioelectronics·G P AndersonK D King
Oct 29, 2000·The Journal of Biological Chemistry·K Els ConrathS Muyldermans
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·H M HaakeG Gauglitz
Jan 29, 2002·Nucleic Acids Research·A W PetersonR M Georgiadis
Mar 29, 2002·International Journal of Cancer. Journal International Du Cancer·Virna Cortez-RetamozoHilde Revets
Sep 19, 2002·Analytical Chemistry·Michael LissElke Prohaska
Jul 18, 2006·Nature Reviews. Microbiology·David H J Bunka, Peter G Stockley
Aug 10, 2006·Chemical Communications : Chem Comm·Abd-Elgawad Radi, Ciara K O'Sullivan
Apr 12, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lisa M Bonanno, Lisa A Delouise
Jun 22, 2007·Analytical and Bioanalytical Chemistry·Teresa MairalCiara K O'Sullivan
Oct 24, 2007·Analytical and Bioanalytical Chemistry·Jeong-O LeeYong Hwan Kim
Apr 9, 2008·Nature Biotechnology·Todd M SquiresScott R Manalis
May 22, 2008·The Analyst·Yongwon JungBong Hyun Chung
Aug 30, 2008·Analytical Chemistry·Johanna-Gabriela WalterThomas Scheper
May 12, 2009·Seminars in Cell & Developmental Biology·Paul J ConroyRichard J O'Kennedy
Nov 26, 2009·Analytical Chemistry·Oara NeumannNaomi J Halas
Jan 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Joonyeong KimVamsi K Yadavalli
May 4, 2011·Biotechnology and Bioengineering·Öznur KökpinarThomas Scheper
Jun 28, 2011·Journal of Immunological Methods·Andrew W Drake, Scott L Klakamp
Oct 27, 2012·Applied Microbiology and Biotechnology·Randy Chi Fai CheungTzi Bun Ng
Jan 23, 2013·The Analyst·Anke K TrillingHan Zuilhof
Sep 5, 2013·Biomicrofluidics·Dohyun Kim, Amy E Herr
Apr 16, 2014·The Analyst·Wenhu ZhouJuewen Liu
Jul 12, 2014·Annual Review of Analytical Chemistry·Günter Gauglitz
Nov 18, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·J A GoodeP A Millner
Jan 1, 2015·Analytical Chemistry·Katharina UrmannEster Segal
Apr 23, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Elena TenenbaumEster Segal
Apr 29, 2015·Biosensors & Bioelectronics·Ailiang Chen, Shuming Yang
Sep 28, 2015·Toxicon : Official Journal of the International Society on Toxinology·Xian ZhangWeihuan Fang
Jun 7, 2016·The Analyst·Nuo DuanZhouping Wang
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Oct 28, 2016·Analytical Chemistry·Yiliang ZhaoSharon M Weiss
Jul 23, 2008·Sensors·Beate StrehlitzRegina Stoltenburg
Jan 14, 2017·Biosensors & Bioelectronics·Rinku ChhasatiaNicolas H Voelcker
Jan 31, 2017·Journal of Biotechnology·Katharina UrmannThomas Scheper

❮ Previous
Next ❯

Citations

Aug 30, 2020·Biosensors·Marc PranteJohanna Walter
Dec 16, 2020·Biomacromolecules·E Eduardo AntunezMarkus Müllner
Jun 5, 2021·ACS Applied Bio Materials·Ofer Prinz SetterEster Segal
Aug 14, 2021·Analytical Biochemistry·Faezeh TaghaviSeyed Mohammad Taghdisi
Oct 26, 2021·ACS Measurement Science Au·Sofia Arshavsky-GrahamEster Segal
Jan 26, 2022·Foodborne Pathogens and Disease·Ece Güven, Reha Onur Azizoglu

❮ Previous
Next ❯

Datasets Mentioned

BETA
MCA1396

Methods Mentioned

BETA
biosensing
SELEX
biosensor
biosensors
immunoprecipitation
scanning electron microscopy
improved
metal
aptasensors
first

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.