AQP0-LTR of the Cat Fr mouse alters water permeability and calcium regulation of wild type AQP0

Biochimica Et Biophysica Acta
Katalin KalmanJ E Hall

Abstract

Aquaporin 0 (AQP0) is the major intrinsic protein of the lens and its water permeability can be modulated by changes in pH and Ca2+. The Cataract Fraser (Cat Fr) mouse accumulates an aberrant AQP0 (AQP0-LTR) in sub-cellular compartments resulting in a congenital cataract. We investigated the interference of AQP0-LTR with normal function of AQP0 in three systems. First, we created a transgenic mouse expressing AQP0 and AQP0-LTR in the lens. Expression of AQP0 did not prevent the congenital cataract but improved the size and transparency of the lens. Second, we measured water permeability of AQP0 co-expressed with AQP0-LTR in Xenopus oocytes. A low expression level of AQP0-LTR decreased the water permeability of AQP0, and a high expression level eliminated its calcium regulation. Third, we studied trafficking of AQP0 and AQP0-LTR in transfected lens epithelial cells. At low expression level, AQP0-LTR migrated with AQP0 toward the cell membrane, but at high expression level, it accumulated in sub-cellular compartments. The deleterious effect of AQP0-LTR on lens development may be explained by lowering water permeability and abolishing calcium regulation of AQP0. This study provides the first evidence that calcium regulation of AQP...Continue Reading

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Citations

Jun 15, 2007·The Journal of Membrane Biology·Richard T MathiasPaul Donaldson
May 30, 2008·The Journal of Biological Chemistry·Katalin KalmanJames E Hall
Oct 17, 2007·The Journal of General Physiology·Karin L Németh-CahalanJames E Hall
Jun 27, 2013·Investigative Ophthalmology & Visual Science·Daniel M ClemensJames E Hall
May 12, 2015·Biochemical and Biophysical Research Communications·S Sindhu KumariKulandaiappan Varadaraj
Nov 23, 2012·Molecular Membrane Biology·Alex C ConnerMatthew T Conner
Apr 23, 2011·Biochimica Et Biophysica Acta·S Sindhu KumariKulandaiappan Varadaraj
Jun 20, 2008·Pharmacology & Therapeutics·Almudena CrookeJesús Pintor
Jul 20, 2018·Investigative Ophthalmology & Visual Science·Irene VorontsovaThomas F Schilling
Apr 27, 2019·Chemical Reviews·James E HallDouglas J Tobias

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