Aquaporin-0 membrane junctions form upon proteolytic cleavage

Journal of Molecular Biology
Tamir GonenThomas Walz

Abstract

Aquaporin-0 (AQP0), previously known as major intrinsic protein (MIP), is the only water pore protein expressed in lens fiber cells. AQP0 is highly specific to lens fiber cells and constitutes the most abundant intrinsic membrane protein in these cells. The protein is initially expressed as a full-length protein in young fiber cells in the lens cortex, but becomes increasingly cleaved in the lens core region. Reconstitution of AQP0 isolated from the core of sheep lenses containing a proportion of truncated protein, produced double-layered two-dimensional (2D) crystals, which displayed the same dimensions as the thin 11 nm lens fiber cell junctions, which are prominent in the lens core. In contrast reconstitution of full-length AQP0 isolated from the lens cortex reproducibly yielded single-layered 2D crystals. We present electron diffraction patterns and projection maps of both crystal types. We show that cleavage of the intracellular C terminus enhances the adhesive properties of the extracellular surface of AQP0, indicating a conformational change in the molecule. This change of function of AQP0 from a water pore in the cortex to an adhesion molecule in the lens core constitutes another manifestation of the gene sharing concep...Continue Reading

References

Dec 1, 1991·The Journal of General Physiology·R T MathiasJ L Rae
Apr 1, 1982·The Journal of Cell Biology·G ZampighiM J Costello
Feb 25, 1980·FEBS Letters·J Kistler, S Bullivant
Apr 14, 1995·The Journal of Biological Chemistry·S M MuldersP Agre
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Oct 6, 1997·The Journal of Membrane Biology·G ChandyJ E Hall
Jun 10, 1998·Journal of Structural Biology·T Walz, N Grigorieff
Aug 12, 1999·The Journal of Membrane Biology·K VaradarajR T Mathias
Jan 6, 2000·Experimental Eye Research·B A MoffatJ M Pope
Mar 4, 2000·The Journal of Biological Chemistry·K L Németh-Cahalan, J E Hall
Jul 4, 2002·The Journal of Physiology·Peter AgreSøren Nielsen
Oct 28, 2003·Investigative Ophthalmology & Visual Science·Lauren E BallKevin L Schey
Apr 23, 2004·Biological Procedures Online·Melanie OhiThomas Walz

❮ Previous
Next ❯

Citations

Jun 15, 2007·The Journal of Membrane Biology·Richard T MathiasPaul Donaldson
Nov 27, 2008·Pflügers Archiv : European journal of physiology·Stéphanie MangenotSimon Scheuring
Jul 30, 2010·Quarterly Reviews of Biophysics·Kutti R Vinothkumar, Richard Henderson
Oct 15, 2010·Biochemistry·Kevin L ScheyAngus C Grey
Sep 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Morten Ø JensenDavid E Shaw
Feb 27, 2013·The Journal of General Physiology·Karin L Németh-CahalanJames E Hall
Feb 1, 2008·Investigative Ophthalmology & Visual Science·Rafal FarjoMuna I Naash
Jun 27, 2013·Investigative Ophthalmology & Visual Science·Daniel M ClemensJames E Hall
Apr 22, 2010·Journal of Lipid Research·Douglas Borchman, Marta C Yappert
Jun 17, 2009·PLoS Biology·Gerhard FischerKarin Lindkvist-Petersson
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Enric ZelaznyFrançois Chaumont
Jan 15, 2013·Experimental Eye Research·Angus C GreyKevin L Schey
May 18, 2013·Experimental Eye Research·Jihong TongThomas J McIntosh
Mar 14, 2009·Differentiation; Research in Biological Diversity·Angus C GreyPaul J Donaldson
Jul 12, 2008·Experimental Eye Research·M J CostelloDorairajan Balasubramanian
Jan 16, 2008·Current Opinion in Structural Biology·Andreas EngelThomas Walz
Oct 9, 2007·Journal of Molecular Biology·Nikolay BuzhynskyySimon Scheuring
Sep 18, 2007·Journal of Structural Biology·Simon ScheuringThomas Walz
Jan 24, 2007·Journal of Molecular Biology·Hector ViadiuThomas Walz
Mar 19, 2016·Experimental Eye Research·Witold K SubczynskiWilliam J O'Brien
Nov 25, 2006·EMBO Reports·Nikolay BuzhynskyySimon Scheuring
Mar 12, 2008·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Robert C Augusteyn
Oct 15, 2013·Biochimica Et Biophysica Acta·Kazutoshi Tani, Yoshinori Fujiyoshi
Feb 4, 2014·Experimental Eye Research·Marija RaguzWitold K Subczynski
Jul 6, 2014·Biophysical Journal·James E Hall, Richard T Mathias
Nov 21, 2013·Biochimica Et Biophysica Acta·Jennie Sjöhamn, Kristina Hedfalk
Nov 5, 2013·Biochimica Et Biophysica Acta·Kevin L ScheyYing Qi
Mar 13, 2014·The International Journal of Biochemistry & Cell Biology·David A ScheiblinMelinda K Duncan
Jan 2, 2008·Journal of Molecular Biology·Tamir GonenThomas Walz
Jul 28, 2016·European Journal of Medical Genetics·Litao QinZhe Liu
May 7, 2016·PloS One·François ChauvignéRoderick Nigel Finn
Dec 15, 2015·Soft Matter·Alice BerthaudStéphanie Mangenot
Oct 28, 2017·International Journal of Molecular Sciences·Jennifer Virginia Roche, Susanna Törnroth-Horsefield
Feb 9, 2018·International Journal of Molecular Sciences·Timothée LalouxFrançois Chaumont
Nov 15, 2018·Cells·Marcelo OzuGabriela Amodeo
Mar 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kulandaiappan VaradarajRichard T Mathias
Apr 1, 2005·American Journal of Physiology. Cell Physiology·Nancy KaufmannJeffrey L Brodsky
May 10, 2008·IUBMB Life·Simeon AndrewsTamir Gonen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.