Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles

Proceedings of the National Academy of Sciences of the United States of America
Sang-Joon ChoB P Jena

Abstract

The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remains largely unknown. Zymogen granules (ZGs), the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a G(alpha)i3 protein. Evidence is presented here that the water channel aquaporin-1 (AQP1) is present in the ZG membrane and participates in rapid GTP-induced vesicular water gating and swelling. Isolated ZGs exhibit low basal water permeability. However, exposure of granules to GTP results in a marked potentiation of water entry. Treatment of ZGs with the known water channel inhibitor Hg2+ is accompanied by a reversible loss in both the basal and GTP-stimulatable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxyl-terminal domain of AQP1 blocks GTP-stimulable swelling of vesicles. Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E M SchwiebertB A Stanton
May 1, 1991·Biophysical Journal·J M FernandezP Verdugo
Oct 1, 1991·The Journal of General Physiology·M J Curran, M S Brodwick
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A Knepper
Jun 10, 1993·Nature·G Alvarez de ToledoJ M Fernández
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·R M HendersonH Oberleithner
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·S W SchneiderB P Jena
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·C DelporteB J Baum
Jun 5, 1997·Nature·T WalzA Engel
Jun 5, 1997·Nature·A ChengA K Mitra
Aug 1, 1997·Current Opinion in Cell Biology·M A Knepper, T Inoue
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·B P JenaK C Sritharan
Jun 17, 1998·The Journal of Biological Chemistry·P AgreM J Borgnia

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Citations

Jun 30, 2005·The Journal of Membrane Biology·M MatsukiH Sugiya
Jul 27, 2006·The Journal of Membrane Biology·Enid A C MacRobbie
Jul 27, 2006·The Journal of Membrane Biology·H Sugiya, M Matsuki
Nov 24, 2007·Pflügers Archiv : European journal of physiology·Kenma NozakiKenichi Ishibashi
Jan 12, 2008·Cell and Tissue Research·Miwako Matsuki-FukushimaHiroshi Sugiya
Aug 18, 2012·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Christophe GoubauKathleen Freson
Sep 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chiara GabbiJan-Ake Gustafsson
Jan 25, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lihua LiA S Verkman
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zuzana BacováVladimir Strbák
May 31, 2008·Molecular Endocrinology·Irina ArnaoutovaY Peng Loh
Jun 25, 2010·Molecular Endocrinology·Victor P FranconeBetty A Eipper
Feb 23, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ouahiba Mesbah-BenmessaoudValérie Grange-Messent
Jul 25, 2009·Journal of the American Society of Nephrology : JASN·Ulla G FriisBoye L Jensen
Aug 15, 2014·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Yasemin KayaLevent Sarikcioglu
Dec 17, 2008·Clinical and Experimental Nephrology·Kenichi IshibashiShintaro Kondo
Nov 16, 2006·Expert Opinion on Therapeutic Targets·Kandiah JeyaseelanE Marelyn Wintour
Oct 27, 2010·Expert Review of Proteomics·María Gómez-LázaroMichael Schrader
Sep 21, 2005·Biology of the Cell·François ChaumontMark J Daniels
Mar 21, 2009·Mass Spectrometry Reviews·Yannick BrunnerJean-Charles Sanchez
Jan 16, 2008·Journal of Cellular and Molecular Medicine·H SugiyaS Hashimoto
Jul 31, 2008·Cellular Microbiology·Andrea L Radtke, Mary X D O'Riordan
Jun 23, 2011·Journal of Cellular and Molecular Medicine·Jin-Sook LeeBhanu P Jena
May 23, 2006·Biochimica Et Biophysica Acta·Yasuko IshikawaYoshiko Nakae
Apr 4, 2006·Biochimica Et Biophysica Acta·Kenichi Ishibashi
Aug 19, 2004·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Constantin Craciun
Mar 21, 2015·The Journal of Membrane Biology·A E Hill, Y Shachar-Hill
Nov 5, 2002·Biophysical Journal·Sang-Joon ChoBhanu P Jena
May 23, 2006·Ultramicroscopy·Bhanu P Jena
Jun 30, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Marie L KellyBhanu P Jena
May 7, 2005·Domestic Animal Endocrinology·Bhanu P Jena
Apr 6, 2005·Biochemical and Biophysical Research Communications·Tomohiro ItohKenichi Ishibashi
Dec 31, 2010·Molecular Membrane Biology·Xin WangKaihe Du
Aug 21, 2013·Biochimica Et Biophysica Acta·Christine Delporte
Jul 24, 2012·Micron : the International Research and Review Journal for Microscopy·Constantin Craciun, Lucian Barbu-Tudoran
Feb 7, 2009·Methods in Cell Biology·Bhanu P Jena
Jul 30, 2016·International Journal of Molecular Sciences·Alessandra PelagalliRosaria Meli
Feb 7, 2009·Methods in Cell Biology·Bhanu P Jena

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