Aquaporin-11 Contributes to TGF-β1-Induced Endoplasmic Reticulum Stress in Human Visceral Adipocytes: Role in Obesity-Associated Inflammation.

Cells
Gema FrühbeckAmaia Rodríguez

Abstract

Aquaporin-11 (AQP11) is expressed in human adipocytes, but its functional role remains unknown. Since AQP11 is an endoplasmic reticulum (ER)-resident protein that transports water, glycerol, and hydrogen peroxide (H2O2), we hypothesized that this superaquaporin is involved in ER stress induced by lipotoxicity and inflammation in human obesity. AQP11 expression was assessed in 67 paired visceral and subcutaneous adipose tissue samples obtained from patients with morbid obesity and normal-weight individuals. We found that obesity and obesity-associated type 2 diabetes increased (p < 0.05) AQP11 mRNA and protein in visceral adipose tissue, but not subcutaneous fat. Accordingly, AQP11 mRNA was upregulated (p < 0.05) during adipocyte differentiation and lipolysis, two biological processes altered in the obese state. Subcellular fractionation and confocal microscopy studies confirmed its presence in the ER plasma membrane of visceral adipocytes. Proinflammatory factors TNF-α, and particularly TGF-β1, downregulated (p < 0.05) AQP11 mRNA and protein expression and reinforced its subcellular distribution surrounding lipid droplets. Importantly, the AQP11 gene knockdown increased (p < 0.05) basal and TGF-β1-induced expression of the ER m...Continue Reading

References

Apr 25, 2000·The Journal of Biological Chemistry·K KishidaY Matsuzawa
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Aug 19, 2005·Molecular and Cellular Biology·Yoshiyuki MorishitaKenichi Ishibashi
Nov 25, 2005·Nature·Gema Frühbeck
May 3, 2006·BMC Biochemistry·Daniel A GorelickPeter Agre
Dec 21, 2006·Biochimica Et Biophysica Acta·Kaya YakataYoshinori Fujiyoshi
Dec 17, 2009·Journal of Visualized Experiments : JoVE·Peter Agre
Jan 1, 2009·The Journal of Medical Investigation : JMI·Kenichi IshibashiYasuko Tanaka
Aug 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Evan W MillerChristopher J Chang
Feb 4, 2011·The Journal of Clinical Endocrinology and Metabolism·Amaia RodríguezGema Frühbeck
Aug 11, 2011·PloS One·Marina R PulidoMaria M Malagón
Feb 9, 2012·The Journal of Biological Chemistry·Margret PaarHeimo Wolinski
Aug 23, 2012·Toxicology and Applied Pharmacology·Maria Julia MarchissioRaúl Alberto Marinelli
Nov 15, 2012·Scientific Reports·Noritaka KawasakiKazunori Imaizumi
Jan 1, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aleksandra RojekSøren Nielsen
Jan 30, 2013·Protein Science : a Publication of the Protein Society·Luisa CalvaneseRomina Oliva
Jun 28, 2013·Adipocyte·James R SkinnerNathan E Wolins
May 30, 2014·Nutrition Research Reviews·Gema FrühbeckAmaia Rodríguez
Jul 11, 2014·Molecular and Cellular Endocrinology·Leire Méndez-GiménezGema Frühbeck
Feb 3, 2016·Biochemical and Biophysical Research Communications·Sachiko WatanabeMariko Hara-Chikuma
Sep 2, 2016·American Journal of Physiology. Renal Physiology·Michael RützlerSøren Nielsen
Nov 12, 2016·Metabolism: Clinical and Experimental·Jesper S HansenKarin Lindkvist-Petersson
May 4, 2018·Frontiers in Molecular Biosciences·Angela TesseGiuseppe Calamita
Dec 19, 2018·Diabetes Care·UNKNOWN American Diabetes Association
Jan 19, 2019·Biochemistry and Biophysics Reports·Yuya HoshinoMasahiro Ikeda
Jul 22, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S EzquerroA Rodríguez
Sep 24, 2019·Redox Biology·Stefano BestettiIria Medraño-Fernandez
Dec 10, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Francesca BaldiniGiuseppe Calamita
Mar 7, 2020·Vitamins and Hormones·Suchun LiWeidong Wang
Apr 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rocío Guzmán-RuizMaría M Malagón
May 11, 2020·Current Opinion in Pharmacology·Amaia RodríguezGema Frühbeck

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Citations

Mar 11, 2021·Biochimie·Giuseppe Calamita, Christine Delporte
Apr 4, 2021·Biochimica Et Biophysica Acta. Biomembranes·Kenichi IshibashiYoshiyuki Morishita
Mar 12, 2021·Endocrine Reviews·Amir AjoolabadyJun Ren
Jun 6, 2021·Redox Biology·Mauro GalliPiotr Zabielski
Jul 31, 2021·Frontiers in Cell and Developmental Biology·Andreina BrunoElisabetta Pace

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Methods Mentioned

BETA
transgenic
gastric bypass
Transfection
PCR
biopsies
confocal microscopy

Software Mentioned

Huygens Essential
SPSS
Primer Express
Quantity One

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