Aquaporin 3 expression in human fetal membranes and its up-regulation by cyclic adenosine monophosphate in amnion epithelial cell culture

Journal of the Society for Gynecologic Investigation
Shengbiao WangMichael G Ross

Abstract

The cell membrane water channel protein aquaporins (AQPs) may be important in regulating the intramembranous (IM) pathway of amniotic fluid (AF) resorption. The objective of the present study was to determine whether aquaporin 3 (AQP3) is expressed in human fetal membranes and to further determine if AQP3 expression in primary human amnion cell culture is regulated by second-messenger cyclic adenosine monophosphate (cAMP). AQP3 expression in human fetal membranes of normal term pregnancy was studied by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC). To determine the effect of cAMP on AQP3 expression, primary human amnion cell cultures were treated in either heat-inactivated medium alone (control), or heat-inactivated medium containing: (1) SP-cAMP, a membrane-permeable and phosphodiesterase resistant cAMP agonist, or (2) forskolin, an adenylate cyclase stimulator. Total RNA was isolated and multiplex real-time RT-PCR employed for relative quantitation of AQP3 expression. We detected AQP3 expression in placenta, chorion, and amnion using RT-PCR. Using IHC, we identified AQP3 protein expression in placenta syncytiotrophoblasts and cytotrophoblasts, chorion cytotrophoblasts, and amnion epit...Continue Reading

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Citations

May 24, 2011·Acta Pharmacologica Sinica·Xiao-yan ShaTong-hui Ma
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang
Sep 12, 2008·Placenta·H LiuE M Wintour
May 23, 2008·European Journal of Pharmacology·Masasi OkahiraKazuyuki Hirano
Nov 28, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christopher Paul CutlerGordon Cramb
Feb 17, 2007·Placenta·M H BeallM G Ross
Nov 18, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Pooja MittalSonia S Hassan
Jul 20, 2007·Reproductive Sciences· Shengbiao WangMichael G Ross
Aug 2, 2007·Obstetrics and Gynecology·Renuka Sekar, Peter R Stone
Apr 4, 2021·Biochimica Et Biophysica Acta. Biomembranes·Jonathan YdeHanne B Moeller
Apr 28, 2021·BMC Cancer·Angeliki AndrikopoulouFlora Zagouri

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