Aquaporin-3 Inhibition Reduces the Growth of NSCLC Cells Induced by Hypoxia

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Shu-Ying HouYe Kuang

Abstract

Non-small cell lung cancer (NSCLC) is the leading cause of death worldwide. Although aquaporin-3 (AQP3) is widely distributed in mammalian tissues and over-expressed in NSCLC cells, there are limited studies on the effects of AQP3 knockdown on NSCLC cells under hypoxic conditions. The CCK-8 assay was used to calculate cell viability. Scratch-wound healing and transwell assays were used to detect cell migration and invasion. Apoptotic cells were measured by the TUNEL assay. mRNA expression levels were calculated via quantitative RT-PCR. Relative protein levels were determined by immunoblot assays. AQP3 knockdown substantially reduced proliferation, migration, and invasion of A549 and NCI-H460 cells under hypoxic conditions. Moreover, AQP3 knockdown clearly induced cell apoptosis. Further analysis identified levels of HIF-1α, VEGF, Raf, phosphor-MEK, and phosphor-ERK, whose activities were significantly attenuated in the AQP3 knockdown group. These findings indicate that AQP3 knockdown retards the growth of NSCLC cells partially through inhibiting HIF-1α/VEGF and Raf/MEK/ERK signalling pathways.

Citations

Feb 13, 2017·Biochemical and Biophysical Research Communications·Guosheng XiongJing Zhang
May 21, 2017·The Journal of Investigative Dermatology·Vivek ChoudharyWendy B Bollag
Nov 7, 2019·International Journal of Cancer. Journal International Du Cancer·Yohan BosséJames D McKay
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Mar 31, 2019·Pathology Oncology Research : POR·Mahdieh-Sadat Moosavi, Yalda Elham
Mar 31, 2021·Reproductive Biology and Endocrinology : RB&E·Yingqi NongFenghua Liu
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Oct 29, 2021·Frontiers in Molecular Biosciences·Guofu LinYuan Xu

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