Aquaporin-4 gene silencing protects injured neurons after early cerebral infarction

Neural Regeneration Research
Zhan-Ping He, Hong Lu

Abstract

Aquaporin-4 regulates water molecule channels and is important in tissue regulation and water transportation in the brain. Upregulation of aquaporin-4 expression is closely related to cellular edema after early cerebral infarction. Cellular edema and aquaporin-4 expression can be determined by measuring cerebral infarct area and apparent diffusion coefficient using diffusion-weighted imaging (DWI). We examined the effects of silencing aquaporin-4 on cerebral infarction. Rat models of cerebral infarction were established by occlusion of the right middle cerebral artery and siRNA-aquaporin-4 was immediately injected via the right basal ganglia. In control animals, the area of high signal intensity and relative apparent diffusion coefficient value on T2-weighted imaging (T2WI) and DWI gradually increased within 0.5-6 hours after cerebral infarction. After aquaporin-4 gene silencing, the area of high signal intensity on T2WI and DWI reduced, relative apparent diffusion coefficient value was increased, and cellular edema was obviously alleviated. At 6 hours after cerebral infarction, the apparent diffusion coefficient value was similar between treatment and model groups, but angioedema was still obvious in the treatment group. These...Continue Reading

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Citations

Aug 17, 2016·International Journal of Molecular Sciences·Heling ChuQiang Dong
Sep 16, 2016·Neural Regeneration Research·Hai-Jun BaoYong-Ping Wu
Jul 27, 2021·Frontiers in Aging Neuroscience·Tao LvXiaohua Zhang

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