Aquaporin-8-facilitated mitochondrial ammonia transport

Biochemical and Biophysical Research Communications
Leandro Raul SoriaGiuseppe Calamita

Abstract

Aquaporin-8 (AQP8) is a membrane channel permeable to water and ammonia. As AQP8 is expressed in the inner mitochondrial membrane of several mammalian tissues, we studied the effect of the AQP8 expression on the mitochondrial transport of ammonia. Recombinant rat AQP8 was expressed in the yeast Saccharomyces cerevisiae. The presence of AQP8 in the inner membrane of yeast mitochondria was demonstrated by subcellular fractionation and immunoblotting analysis. The ammonia transport was determined in isolated mitochondria by stopped flow light scattering using formamide as ammonia analog. We found that the presence of AQP8 increased by threefold mitochondrial formamide transport. AQP8-facilitated mitochondrial formamide transport in rat native tissue was confirmed in liver (a mitochondrial AQP8-expressing tissue) vs. brain (a mitochondrial AQP8 non-expressing tissue). Comparative studies indicated that the AQP8-mediated mitochondrial movement of formamide was markedly higher than that of water. Together, our data suggest that ammonia diffusional transport is a major function for mitochondrial AQP8.

References

Aug 28, 1997·Biochemical and Biophysical Research Communications·K IshibashiS Sasaki
Dec 31, 1997·The Journal of Biological Chemistry·Y KoyamaI Kihara
Dec 6, 1997·Biochemical and Biophysical Research Communications·T MaA S Verkman
Nov 13, 2001·American Journal of Physiology. Renal Physiology·M L ElkjaerS Nielsen
Feb 5, 2002·European Journal of Cell Biology·G CalamitaM Svelto
Apr 5, 2002·The Journal of Biological Chemistry·Robert C HuebertNicholas F LaRusso
Aug 27, 2003·The Journal of Biological Chemistry·Raul A MarinelliNicholas F LaRusso
Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Domenico FerriGiuseppe Calamita
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Landon S KingPeter Agre
Mar 8, 2005·The Journal of Biological Chemistry·Giuseppe CalamitaMaria Svelto
Jun 14, 2005·Biology of the Cell·Kun LiuPeter Agre
Jun 22, 2005·Biology of the Cell·Nina PetterssonStefan Hohmann
Jul 1, 2005·Pflügers Archiv : European journal of physiology·Lars M HolmThomas Zeuthen
Apr 21, 2006·The Journal of Biological Chemistry·Baoxue YangA S Verkman
Apr 21, 2006·American Journal of Physiology. Cell Physiology·Baoxue YangA S Verkman
Aug 29, 2006·Biochimica Et Biophysica Acta·Giuseppe CalamitaMaria Svelto
Nov 16, 2006·The Journal of Biological Chemistry·Gerd P BienertThomas P Jahn
Dec 26, 2006·The Journal of Biological Chemistry·Sapar M SaparovPeter Pohl
Jan 11, 2007·The Journal of Endocrinology·Giuseppe CalamitaFernando Goglia
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
Jun 16, 2007·Cellular and Molecular Life Sciences : CMLS·B Wu, E Beitz
Aug 2, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Giuseppe CalamitaMaria Svelto
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Patrizia GenaGiuseppe Calamita

❮ Previous
Next ❯

Citations

Jul 10, 2013·Antioxidants & Redox Signaling·Raul A Marinelli, Maria J Marchissio
Nov 5, 2010·American Journal of Physiology. Renal Physiology·I David Weiner, Jill W Verlander
Nov 15, 2014·Plant Physiology and Biochemistry : PPB·Vito ScaleraGiuseppe Calamita
Jul 11, 2014·Molecular and Cellular Endocrinology·Leire Méndez-GiménezGema Frühbeck
Jan 23, 2016·Anatomical Science International·Toshiyuki MatsuzakiKuniaki Takata
Apr 11, 2012·Molecular Aspects of Medicine·Piero Portincasa, Giuseppe Calamita
Jan 31, 2012·Journal of Molecular and Cellular Cardiology·David Garcia-DoradoIgnasi Barba
Sep 8, 2015·Current Opinion in Structural Biology·Stefan Kreida, Susanna Törnroth-Horsefield
Jan 10, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Leandro R SoriaRaúl A Marinelli
Jul 2, 2015·Histochemistry and Cell Biology·Françoise GregoireChristine Delporte
Aug 23, 2012·Toxicology and Applied Pharmacology·Maria Julia MarchissioRaúl Alberto Marinelli
Sep 24, 2013·Biochemical and Biophysical Research Communications·Shu-Juan ZhuShan-Quan Sun
Dec 24, 2011·Biochimica Et Biophysica Acta·Maria AgemarkPer Kjellbom
Jul 22, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Sara M MolinasRaúl A Marinelli
Apr 17, 2012·Biochimica Et Biophysica Acta·Alexandra V KareyevaAndrei D Vinogradov
Mar 15, 2016·Antioxidants & Redox Signaling·Grazia Tamma, Giovanna Valenti
Sep 14, 2016·Expert Opinion on Therapeutic Patents·Graça Soveral, Angela Casini
Jul 15, 2016·International Journal of Molecular Sciences·Raquel L BernardinoGiuseppe Calamita
Jan 6, 2018·International Journal of Molecular Sciences·Inês Vieira da SilvaGraça Soveral
Nov 23, 2017·Scientific Reports·Rafael Hoyos-ManchadoVíctor A Tallada
Jul 24, 2018·Physiological Reports·Cecilia Y CheungRobert A Brace
Feb 20, 2018·BMC Structural Biology·Andreas KirschtUrban Johanson
Sep 27, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hiroko Nishimura, Yimu Yang
Mar 25, 2020·Archives of Toxicology·Wing-Kee Lee, Frank Thévenod
May 25, 2012·American Journal of Physiology. Renal Physiology·Sara M MolinasRaúl A Marinelli
Mar 15, 2013·American Journal of Physiology. Cell Physiology·R Ryan GeyerWalter F Boron
Feb 24, 2018·Bioscience Reports·Mandika ChetryXueqiong Zhu
May 26, 2018·International Journal of Molecular Sciences·Luisa CalvaneseRomina Oliva
Apr 3, 2019·International Journal of Molecular Sciences·Mohammed Abir-AwanRoslyn M Bill
Mar 23, 2017·IUBMB Life·Mauro DanielliRaúl A Marinelli
Nov 25, 2020·Cellular and Molecular Life Sciences : CMLS·Inês Vieira da SilvaGraça Soveral
Mar 5, 2021·Bio-protocol·Patrizia GenaGiuseppe Calamita
Apr 24, 2021·Redox Biology·Christina KrügerMatthias Elsner
Jul 23, 2021·Biotechnology and Bioengineering·Alejo M CapiglioniRaúl A Marinelli
Jan 14, 2017·The American Journal of Pathology·François BlachierDaniel Tomé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Gastrointestinal and Liver Physiology
M Cecilia LaroccaRaúl A Marinelli
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Leandro Raul SoriaRaúl A Marinelli
The Journal of Biological Chemistry
Sapar M SaparovPeter Pohl
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Domenico FerriGiuseppe Calamita
© 2021 Meta ULC. All rights reserved