Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones.

Reproduction : the Official Journal of the Society for the Study of Fertility
Nuria Pastor-SolerSylvie Breton

Abstract

Fluid and solute transport across the epithelium of the male excurrent duct is important for sperm maturation and storage. Aquaporin 9 (AQP9), which allows permeation of water and neutral solutes, is abundant throughout the male reproductive tract, where it is expressed at the apical membrane of rat epididymal principal cells as early as at 1 week of age. We evaluated the effect of neonatal exposure to: 1) a GNRH antagonist (GNRHa); 2) diethylstilbestrol (DES); 3) ethinyl estradiol (EE); 4) DES plus testosterone (DES+TE); and 5) the anti-androgen flutamide on AQP9 expression in the epididymis of peripubertal rats. Control groups received the vehicle alone. In 25-day-old rats, quantification of the mean pixel intensity of immunofluorescence-stained sections showed a significant decrease in AQP9 staining in the apical membrane of epididymal principal cells after treatments with GNRHa, DES, or flutamide, compared to controls. These results were confirmed by western blotting. While EE induced a marked decrease in AQP9 levels by western blotting, the decrease in AQP9-associated fluorescence was not significant compared to controls. DES+TE-treated rats showed levels of AQP9 protein similar to controls, indicating maintenance of AQP9 ...Continue Reading

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Citations

May 11, 2010·Reproduction, Fertility, and Development·Alvaro E Domínguez-RebolledoJ Julián Garde
Oct 12, 2010·Biology of Reproduction·Avenel JosephRex A Hess
Aug 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Winnie W C ShumSylvie Breton
Jan 15, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A RodríguezG Frühbeck
Oct 22, 2013·Reproduction, Fertility, and Development·S Arrighi
Mar 20, 2012·Cell and Tissue Research·Clémence BelleannéeRobert Sullivan
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang
Jul 6, 2013·Reproduction in Domestic Animals = Zuchthygiene·S Arrighi, M Aralla
May 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·B C SchimmingR F Domeniconi
Jun 13, 2015·Journal of Molecular Endocrinology·Fernanda N CavalcantiCatarina S Porto
Feb 7, 2015·American Journal of Physiology. Renal Physiology·Carol M Herak-KrambergerIvan Sabolić
Feb 4, 2011·The Journal of Clinical Endocrinology and Metabolism·Amaia RodríguezGema Frühbeck
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·M YesteN Prieto-Martínez
Nov 16, 2018·Journal of Molecular Histology·Nur Siti Khadijah RamliNaguib Salleh
Apr 4, 2017·Reproduction in Domestic Animals = Zuchthygiene·B C SchimmingR F Domeniconi
Jan 15, 2021·Differentiation; Research in Biological Diversity·Rex A HessBarry T Hinton
Feb 13, 2020·Reproductive Toxicology·Temidayo S Omolaoye, Stefan S du Plessis
Mar 22, 2021·Animal Reproduction Science·Nicola MirabellaCaterina Squillacioti
Nov 13, 2020·Differentiation; Research in Biological Diversity·Gerald R CunhaLaurence S Baskin
Jun 3, 2021·International Journal of Molecular Sciences·Agata Wawrzkiewicz-JałowieckaGraça Soveral
Jul 25, 2021·Animal Reproduction Science·Patrycja Oberska, Katarzyna Michałek
May 5, 2021·Reproduction, Fertility, and Development·R R MillerB Nixon
Dec 6, 2011·Reproductive Biology·Mariusz T SkowronskiAgnieszka Skowronska

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