Aquaporin tetramer composition modifies the function of tobacco aquaporins.

The Journal of Biological Chemistry
Beate OttoRalf Kaldenhoff

Abstract

Heterologous expression in yeast cells revealed that NtAQP1, a member of the so-called PIP1 aquaporin subfamily, did not display increased water transport activity in comparison with controls. Instead, an increased CO(2)-triggered intracellular acidification was observed. NtPIP2;1, which belongs to the PIP2 subfamily of plant aquaporins, behaved as a true aquaporin but lacked a CO(2)-related function. Results from split YFP experiments, protein chromatography, and gel electrophoresis indicated that the proteins form heterotetramers when coexpressed in yeast. Tetramer composition had effects on transport activity as demonstrated by analysis of artificial heterotetramers with a defined proportion of NtAQP1 to NtPIP2;1. A single NtPIP2;1 aquaporin in a tetramer was sufficient to significantly increase the water permeability of the respective yeast cells. With regard to CO(2)-triggered intracellular acidification, a cooperative effect was observed, where maximum rates were measured when the tetramer consisted of NtAQP1 aquaporins only. The results confirm the model of an aquaporin monomer as a functional unit for water transport and suggest that, for CO(2)-related transport processes, a structure built up by the tetramer is the bas...Continue Reading

References

Jul 15, 1987·European Journal of Biochemistry·J Ricard, A Cornish-Bowden
Aug 1, 1994·The Plant Journal : for Cell and Molecular Biology·W KammerloherA R Schäffner
Aug 1, 1993·Protein Science : a Publication of the Protein Society·V BaudinC Poyart
Jun 5, 1997·Nature·A ChengA K Mitra
Mar 5, 1998·The American Journal of Physiology·N L NakhoulW F Boron
Jun 6, 1998·Journal of Structural Biology·J B HeymannA Engel
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·A BielaR Kaldenhoff
Apr 5, 2000·Biochimica Et Biophysica Acta·I JohanssonP Kjellbom
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·M Marin-OlivierT Gaude
Jul 18, 2001·American Journal of Physiology. Renal Physiology·N L NakhoulL L Hamm
Apr 24, 2002·The Plant Cell·Franka SiefritzRalf Kaldenhoff
Jul 4, 2002·The Journal of Physiology·Gordon J CooperWalter F Boron
Dec 13, 2003·The Plant Cell·Karolina FetterFrançois Chaumont
Aug 18, 2004·Plant Physiology·Menachem MoshelionFrançois Chaumont
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Keren Bracha-DroriNir Ohad
Aug 25, 2005·Methods in Molecular Biology·Barry Panaretou, Peter Piper
Jun 1, 2006·Acta Physiologica·R Kaldenhoff, M Fischer
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Jaume FlexasRalf Kaldenhoff
Oct 3, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V EndewardG Gros
Feb 20, 2007·Journal of Structural Biology·Yi WangEmad Tajkhorshid
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Enric ZelaznyFrançois Chaumont
Sep 29, 2007·Nature Methods·Michael T KirberJohn F Keaney
Oct 31, 2007·FEBS Letters·Adam Bertl, Ralf Kaldenhoff
Sep 24, 2008·The Plant Journal : for Cell and Molecular Biology·Enric ZelaznyFrançois Chaumont
Mar 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Raif Musa-AzizWalter F Boron
May 19, 2009·Biochemistry·Line StrandTorgeir Holen

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Citations

Aug 3, 2011·Pflügers Archiv : European journal of physiology·Marcela Herrera, Jeffrey L Garvin
Nov 18, 2011·Molecular Plant-microbe Interactions : MPMI·Alfonso Navarro-RódenasAsunción Morte
May 10, 2013·Molecular Plant-microbe Interactions : MPMI·Alfonso Navarro-RódenasAsunción Morte
Jan 11, 2014·Plant & Cell Physiology·Izumi C MoriMaki Katsuhara
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Agustín YaneffGabriela Amodeo
Apr 8, 2014·Plant Physiology and Biochemistry : PPB·Malika AyadiKhaled Masmoudi
Aug 2, 2014·Plant Molecular Biology·Robert B HeinenFrançois Chaumont
Jul 30, 2014·Applied Microbiology and Biotechnology·Anita EmmerstorferHarald Pichler
Oct 10, 2014·Current Opinion in Plant Biology·Lionel VerdoucqChristophe Maurel
Sep 13, 2014·Applied Microbiology and Biotechnology·Uwe Nehls, Sandra Dietz
Nov 29, 2014·Critical Reviews in Biotechnology·Ashish Kumar SrivastavaLam-Son Phan Tran
Nov 4, 2015·FEBS Letters·Agustín YaneffGabriela Amodeo
Jan 21, 2016·Plant Signaling & Behavior·Hongtao Ji, Hansong Dong
Jan 8, 2013·Trends in Plant Science·Charles HachezFrançois Chaumont
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Jaume FlexasCharles R Warren
Feb 4, 2012·Current Opinion in Plant Biology·Ralf Kaldenhoff
Apr 9, 2015·Plant, Cell & Environment·Anastassia BoudichevskaiaRalf Kaldenhoff
Apr 11, 2015·Plant, Cell & Environment·Alfonso Navarro-RóDenasJanusz J Zwiazek
Dec 3, 2013·The Journal of Membrane Biology·Juliana Perez Di GiorgioNicolás Daniel Ayub
May 14, 2011·The Plant Journal : for Cell and Molecular Biology·Marlies HeckwolfRalf Kaldenhoff
Jan 8, 2013·Biology of the Cell·Xiaodong DingGiuseppe Calamita
Dec 14, 2011·Plant, Cell & Environment·Norbert UehleinRalf Kaldenhoff
Aug 14, 2012·Plant, Cell & Environment·John R Evans, Susanne von Caemmerer
Sep 7, 2016·Plant Science : an International Journal of Experimental Plant Biology·Margaret M Barbour, Brent N Kaiser
Mar 31, 2016·Biophysical Journal·Cintia JozefkowiczKarina Alleva
Aug 19, 2015·Protein Expression and Purification·Lili WangQingsong Lin
Nov 5, 2014·Plant, Cell & Environment·Guowei LiVéronique Santoni

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