Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway

Oncotarget
Guanqun Chao, Shuo Zhang

Abstract

Our research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models' colon so as to find novel pathogenesisof IBS. The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05). 18 adult female SD big rats were divided into three groups:the rats in control group were normal rats,the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8. Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.

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Citations

Feb 9, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Dongrong ZhuJianguang Luo
Mar 6, 2018·Frontiers in Physiology·Rosaria MeliAlessandra Pelagalli
Aug 11, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yu ZhanShiyu Tang
Apr 30, 2019·Clinical and Translational Gastroenterology·Michael CamilleriIrene Busciglio
Jan 15, 2021·European Journal of Medical Research·Guanqun ChaoShuo Zhang
May 3, 2019·Clinical and Translational Gastroenterology·Michael CamilleriIrene Busciglio
Jun 11, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Mohamed ElbadawiThomas Efferth
Nov 26, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Li ZhaoHongfang Li

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Methods Mentioned

BETA
PCR
chips

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