PMID: 16830486Jul 13, 2006Paper

Aqueous chromatography system using temperature-responsive polymer-modified stationary phases

Journal of Separation Science
Eri Ayano, H Kanazawa

Abstract

Extensive research has been carried out on functional polymers which are currently playing important roles in various fields such as medicine and engineering. Such functional polymers which respond to various kinds of stimuli are termed 'intelligent materials'. Poly(N-isopropylacrylamide) (PNIPAAm), a temperature-responsive polymer, was utilized as a chromatography column matrix modifier for a novel chromatographic approach in which only aqueous media are used as a mobile phase. The ability of the developed temperature-responsive chromatography system to separate solutes without using an organic solvent is advantageous from the point of view of maintaining the structure and activity of bioactive compounds. Recently, we designed and synthesized a new pH- and temperature-responsive copolymer as a representative of such environment-responsive polymers and grafted it onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for separation systems based on a new concept, according to which the properties of the stationary phase surface are altered by external stimuli such as pH and temperature. This chromatography system utilizing the PNIPAAm copolymer is very useful for the separation of bioactive substan...Continue Reading

References

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Citations

Mar 28, 2009·Journal of the Royal Society, Interface·Kenichi NagaseT Okano
Apr 26, 2015·Analytical and Bioanalytical Chemistry·Rosa A LorenzoCarmen Alvarez-Lorenzo
Jul 8, 2015·Journal of Chromatography. a·Alexandros LamprouMassimo Morbidelli
Dec 8, 2011·Macromolecular Bioscience·Kenichi NagaseT Okano
Mar 2, 2013·Chemical Society Reviews·Debashish RoyBrent S Sumerlin
Dec 15, 2020·Journal of Chromatography. a·Javier González-SálamoJavier Hernández-Borges
Nov 10, 2020·Analytica Chimica Acta·Kenichi Nagase, H Kanazawa
Apr 13, 2010·ACS Applied Materials & Interfaces·Kenichi NagaseT Okano

Related Concepts

Oligo(N-isopropylacrylamide)
Acrylic Resins
Chromatography
High Pressure Liquid Chromatography Procedure
Environmental Pollutants
Hydrogen-Ion Concentration
Polypeptides
Polymers
Proline
Thermodynamics

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