Aqueous-filled polymer microcavity arrays: versatile & stable lipid bilayer platforms offering high lateral mobility to incorporated membrane proteins

The Analyst
Hajra BasitTia E Keyes

Abstract

A key prerequisite in an ideal supported lipid bilayer based cell membrane model is that the mobility of both the lipid matrix and its components are unhindered by the underlying support. This is not trivial and with the exception of liposomes, many of even the most advanced approaches, although accomplishing lipid mobility, fail to achieve complete mobility of incorporated membrane proteins. This is addressed in a novel platform comprising lipid bilayers assembled over buffer-filled, arrays of spherical cap microcavities formed from microsphere template polydimethoxysilane. Prior to bilayer assembly the PDMS is rendered hydrophilic by plasma treatment and the lipid bilayer prepared using Langmuir Blodgett assembly followed by liposome/proteoliposome fusion. Fluorescence Lifetime Correlation Spectroscopy confirmed the pore suspended lipid bilayer exhibits diffusion coefficients comparable to free-standing vesicles in solution. The bilayer modified arrays are highly reproducible and stable over days. As the bilayers are suspended over deep aqueous reservoirs, reconstituted membrane proteins experience an aqueous interface at both membrane interfaces and attain full lateral mobility. Their utility as membrane protein platforms wa...Continue Reading

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Citations

Mar 15, 2016·Analytical Chemistry·Ahmad KenaanAnne Magali Charrier
Jun 15, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·H BasitT E Keyes
Jul 3, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomonori NomotoMasanori Fujinami
Aug 22, 2020·Chemical Communications : Chem Comm·Guilherme B BerselliTia E Keyes
Jun 15, 2021·ACS Applied Materials & Interfaces·Guilherme B BerselliTia E Keyes
May 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Sivaramakrishnan RamaduraiTia E Keyes

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