Aqueous Humor Dynamics of the Brown-Norway Rat

Investigative Ophthalmology & Visual Science
Kayla R FicarrottaChristopher L Passaglia

Abstract

The study aimed to provide a quantitative description of aqueous humor dynamics in healthy rat eyes. One eye of 26 anesthetized adult Brown-Norway rats was cannulated with a needle connected to a perfusion pump and pressure transducer. Pressure-flow data were measured in live and dead eyes by varying pump rate (constant-flow technique) or by modulating pump duty cycle to hold intraocular pressure (IOP) at set levels (modified constant-pressure technique). Data were fit by the Goldmann equation to estimate conventional outflow facility (C) and unconventional outflow rate (Fun). Parameter estimates were respectively checked by inserting a shunt of similar conductance into the eye and by varying eye hydration methodology. Rat IOP averaged 14.6 ± 1.9 mm Hg at rest. Pressure-flow data were repeatable and indistinguishable for the two perfusion techniques, yielding C = 0.023 ± 0.002 μL/min/mm Hg and Fun = 0.096 ± 0.024 μL/min. C was similar for live and dead eyes and increased upon shunt insertion by an amount equal to shunt conductance, validating measurement accuracy. At 100% humidity Fun dropped to 0.003 ± 0.030 μL/min. Physiological washout was not observed (-0.35 ± 0.65%/h), and trabecular anatomy looked normal. Rat aqueous humo...Continue Reading

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Citations

Aug 28, 2019·Cornea·Claes H DohlmanEleftherios I Paschalis
Nov 27, 2019·The Journal of Physiology·Kayla R Ficarrotta, Christopher L Passaglia
May 15, 2020·Investigative Ophthalmology & Visual Science·Andrew J FeolaC Ross Ethier
Nov 12, 2019·Frontiers in Bioengineering and Biotechnology·Joseph M SherwoodDarryl R Overby
Jan 12, 2020·Scientific Reports·Kayla R FicarrottaChristopher L Passaglia
Aug 15, 2021·Experimental Eye Research·Christina M NicouChristopher L Passaglia
Jan 19, 2022·Investigative Ophthalmology & Visual Science·Jessica V JasienBrian C Samuels

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