Aqueous humor metabolite profile of pseudoexfoliation glaucoma is distinctive.

Molecular Omics
Ciara MyerSanjoy K Bhattacharya

Abstract

Pseudoexfoliation (PEX) is a known cause of secondary open angle glaucoma. PEX glaucoma is associated with structural and metabolic changes in the eye. Despite similarities, PEX and primary open angle glaucoma (POAG) may have differences in the composition of metabolites. We analyzed the metabolites of the aqueous humor (AH) of PEX subjects sequentially first using nuclear magnetic resonance (1H NMR: HSQC and TOCSY), and subsequently with liquid chromatography tandem mass spectrometry (LC-MS/MS) implementing isotopic ratio outlier analysis (IROA) quantification. The findings were compared with previous results for POAG and control subjects analyzed using identical sequential steps. We found significant differences in metabolites between the three conditions. Principle component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) indicated clear grouping based on the metabolomes of the three conditions. We used machine learning algorithms and a percentage set of the data to train, and utilized a different or larger dataset to test whether a trained model can correctly classify the test dataset as PEX, POAG or control. Three different algorithms: linear support vector machines (SVM), deep learning, and a neura...Continue Reading

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Citations

Dec 18, 2020·Current Opinion in Ophthalmology·Ursula Schlötzer-Schrehardt, Chiea Chuen Khor
Feb 6, 2021·Journal of Clinical Medicine·Ram B KhattriTerence E Ryan
May 7, 2021·Investigative Ophthalmology & Visual Science·Ying WangChen-Wei Pan
Jun 1, 2021·Frontiers in Molecular Biosciences·Diana Anna DmuchowskaMichal Ciborowski
Jun 17, 2021·Expert Review of Proteomics·Luheng LyuSanjoy Bhattacharya

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Datasets Mentioned

BETA
ST001284

Methods Mentioned

BETA
NMR
nuclear magnetic resonance

Software Mentioned

PLS
MetaboAnalyst
DA
GraphPad Prism
MetsScape
MetScape
Cytoscape
GARP4
Clusterfinder
RapidMiner

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