Aqueous humor myocilin protein levels in normal, genetic carriers, and glaucoma Beagles

Veterinary Ophthalmology
E O MacKayK N Gelatt

Abstract

The gene (myocilin: MYOC) has been attributed to be involved in over 6% of inherited types of human glaucoma, the highest correlation for any gene to date. This study determines myocilin protein levels in the aqueous humor (AH) of normal laboratory quality, genetic carrier (offspring of normal laboratory quality and POAG Beagles), and primary open angle glaucoma (POAG) Beagles. Eighteen dogs were used and classified as either normal, carrier or having mild, moderate or advanced POAG. A 0.1 mL sample of AH was drawn from the anterior chamber of each dog in the study and frozen on dry ice. A modified Coomassie stain and Western blot, using a polyclonal rabbit antihuman myocilin antibody (Santa Cruz Biotechnologies, Santa Cruz, CA), was run on each sample to compare the myocilin levels. A purified human trabecular meshwork excreted myocilin protein sample was used as a control (Alcon Research Laboratories, Fort Worth, TX) and its band/densitometry measurement was defined as one unit of myocilin for comparisons. Comparisons of AH myocilin levels differed among normal laboratory quality, genetic carrier, and POAG Beagles at different stages of the disease. In the normal laboratory, Beagles the AH myocilin measured 0.817 +/- 0.075 un...Continue Reading

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Citations

Oct 12, 2010·Experimental Eye Research·Zachary T ReschMichael P Fautsch
Sep 23, 2008·Experimental Eye Research·Zachary T Resch, Michael P Fautsch
Oct 13, 2015·The Veterinary Clinics of North America. Small Animal Practice·Stefano Pizzirani
Aug 19, 2015·The Veterinary Clinics of North America. Small Animal Practice·András M Komáromy, Simon M Petersen-Jones
Jul 22, 2008·Veterinary Ophthalmology·E O MacKayK N Gelatt
Nov 17, 2010·Experimental Eye Research·Rachida A BouhenniDeepak P Edward
Jan 24, 2019·Pharmaceutical Research·Mitalee TamhaneVeena Viswanath

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