Aqueous matrix compositions and pH influence feline calicivirus inactivation by high pressure processing

Journal of Food Protection
David H Kingsley, Haiqiang Chen

Abstract

The individual effects of pH (pH 3 to 8), NaCl (0 to 21%), sucrose (0 to 70%), and whey protein (0 to 2%) on pressure resistance of feline calicivirus (FCV) in Dulbecco's modified Eagle medium with 10% fetal bovine serum were determined. At pH 3 through 8, the virus was more resistant to pressure at a pH of < or = 5.2. For FCV samples with sucrose (up to 40%) or NaCl (up to 12%), the amount of FCV inactivated by pressure was inversely proportional to the sucrose or NaCl concentration. For example, a treatment of 250 MPa at 20 degrees C for 5 min reduced the FCV titer by 5.1 log PFU/ml without added sucrose and by 0.9 log PFU/ml with 40% sucrose. Reduced pressure sensitivity with increasing NaCl and sucrose concentrations was not a simple function of water activity. Different PFU reductions were observed for NaCl and sucrose samples with equivalent water activity. When protein at concentrations up to 2% did not provide a protective effect. The combined effect of NaCl and sucrose at 4 and 20 degrees C on pressure resistance of FCV also was examined. When both NaCl and sucrose were added to the FCV stock, they had an additive effect on increasing the pressure resistance of FCV. The individual (6% NaCl or 20% sucrose) and combined ...Continue Reading

References

Oct 17, 2002·Journal of Food Protection·David H KingsleyGary P Richards
Aug 21, 2003·International Journal of Food Microbiology·Haiqiang Chen, Dallas G Hoover
Jan 14, 2004·The Journal of General Virology·Erwin DuizerMary K Estes
Mar 24, 2004·Journal of Food Protection·Haiqiang ChenDallas G Hoover
Apr 7, 2004·Applied and Environmental Microbiology·Adriana Molina-HöppnerMichael G Gänzle
Sep 29, 2004·Biophysical Chemistry·Jose L R SantosCarlos F S Bonafe
Jan 11, 2005·Applied and Environmental Microbiology·Kevin R CalciDavid H Kingsley
Jan 19, 2006·Journal of Food Protection·Haiqiang ChenDallas G Hoover
Mar 17, 2006·Biochimica Et Biophysica Acta·Dietrich KnorrRoman Buckow
Jun 8, 2007·Emerging Infectious Diseases·Timothy M StraubCheryl A Nickerson
Aug 1, 2005·Journal of Food Protection·David H KingsleyDallas G Hoover

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Citations

Aug 18, 2009·Journal of Food Protection·Stephen F GroveThomas Ross
Jul 19, 2015·Applied and Environmental Microbiology·Elbashir AraudJianrong Li
Jul 31, 2016·Applied and Environmental Microbiology·Fangfei LouJianrong Li
Mar 7, 2017·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Mamoru Noda
Jun 2, 2016·Critical Reviews in Food Science and Nutrition·Shruti ShuklaMyunghee Kim
Apr 18, 2015·Annual Review of Food Science and Technology·Fangfei LouJianrong Li
Mar 20, 2010·Critical Reviews in Food Science and Nutrition·Peter J Taormina
Feb 16, 2013·Food and Environmental Virology·David H Kingsley
Apr 30, 2013·Food and Environmental Virology·Christina JarkeThiemo Albert
Nov 28, 2013·Pharmaceuticals·Raymond Nims, Mark Plavsic
Oct 27, 2016·Food and Environmental Virology·Eva EmmothFrederik Widén
Nov 3, 2010·Foodborne Pathogens and Disease·Gloria SánchezDolores Rodrigo
Jul 11, 2020·Journal of Food Protection·Hany AhmedJenni Korhonen
Jan 27, 2021·Foods·Alexander Govaris, Andreana Pexara
Jan 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Kirsten A HirneisenKalmia E Kniel
Nov 16, 2016·International Journal of Food Microbiology·Robert F SidoHaiqiang Chen
Jun 1, 2021·Comprehensive Reviews in Food Science and Food Safety·Kemal AganovicVolker Heinz

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