Aqueous normal-phase retention of nucleotides on silica hydride columns

Journal of Chromatography. a
Joseph J PesekReinhard I Boysen

Abstract

The use of silica hydride-based stationary phases for the retention and analysis of nucleotides has been investigated. Both reversed-phase columns with a hydride surface underneath as well as those with an unmodified or a minimally modified hydride material were tested. With these systems, an aqueous normal-phase mode was used with high organic content mobile phases in combination with an additive to control pH for the retention of the hydrophilic nucleotides. Isocratic and gradient elution formats have been used to optimize separations for mixtures containing up to seven components. All conditions developed are suitable for methods that utilize mass spectrometry detection.

References

Apr 28, 2005·Current Protein & Peptide Science·Jaroslav SebestíkIvan Stibor
Jul 17, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael KronIsaac Matus
Mar 4, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Karen-Marie OlesenKjeld Schmiegelow

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Citations

May 11, 2010·Journal of the American Society for Mass Spectrometry·Benjamin P Bowen, Trent R Northen
Feb 19, 2011·Analytical Chemistry·Oscar YanesGary Siuzdak
Feb 1, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S StudzińskaB Buszewski
Mar 25, 2014·Journal of Pharmaceutical and Biomedical Analysis·Joseph J PesekJoshua E Young
Sep 18, 2015·Journal of Separation Science·Lydia KisleyChristy F Landes
Sep 6, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jing GeQing X Li
Mar 20, 2012·Journal of Pharmaceutical and Biomedical Analysis·Joseph J PesekAndy Dang
Jul 23, 2013·Journal of Separation Science·Yuanzhong YangMilton T W Hearn
Jun 24, 2010·Journal of Separation Science·Joseph J PesekKavita V Prajapati
Oct 6, 2011·Journal of Separation Science·Gary J Patti
Oct 20, 2011·Journal of Separation Science·Joseph J PesekSteven M Fischer
May 29, 2013·Journal of Separation Science·Joseph J PesekAmy M Kim
Jul 14, 2010·Mass Spectrometry Reviews·Robert S JansenJos H Beijnen
Jun 9, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun WangM S Yang
Sep 23, 2014·Journal of Chromatographic Science·Endler M Borges
Aug 18, 2010·Analytical Chemistry·Jorge E Gomez, Junior E Sandoval

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