AR-PDEF pathway promotes tumour proliferation and upregulates MYC-mediated gene transcription by promoting MAD1 degradation in ER-negative breast cancer

Molecular Cancer
Lu CaoYun Niu

Abstract

Androgen receptor (AR) is expressed in 60%~ 70% oestrogen receptor (ER)-negative breast cancer (BC) cases and promotes the growth of this cancer subtype. Expression of prostate-derived Ets factor (PDEF), a transcription factor, is highly restricted to epithelial cells in hormone-regulated tissues. MYC and its negative regulator MAD1 play an important role in BC progression. Previously, we found that PDEF expression is strongly correlated with AR expression. However, the relationship between AR and PDEF and the function of PDEF in ER-negative BC proliferation are unclear. AR and PDEF expression in ER-negative BC tissues and cell lines was determined by performing immunohistochemistry or western blotting. Protein expression levels and location were analysed by performing western blotting, RT-qPCR and immunofluorescence staining. Co-immunoprecipitation and chromatin immunoprecipitation assays were performed to validate the regulation of AR-PDEF-MAD1-MYC axis. Moreover, the effect of AR and PDEF on BC progression was investigated both in vitro and in vivo. We found that PDEF was overexpressed in ER-negative BC tissues and cell lines and appeared to function as an oncogene. PDEF expression levels were strongly correlated with AR exp...Continue Reading

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Citations

Jan 17, 2020·Journal of Cellular and Molecular Medicine·Yan LiuLiming Chen
Jun 7, 2019·Journal of Experimental & Clinical Cancer Research : CR·Cong XuYun Niu
Aug 18, 2020·Science China. Life Sciences·Song-Yang WuYi-Zhou Jiang
Feb 23, 2020·Virchows Archiv : an International Journal of Pathology·Jing LiuYun Niu
Jun 18, 2020·Cancer Management and Research·Ting YeJinbo Liu
Jun 18, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yu-Hua LinXiong-Biao Wang
Jul 1, 2021·Journal of Cellular and Molecular Medicine·Ting YeJinbo Liu

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Methods Mentioned

BETA
protein assay
immunoprecipitation
ChIP
PCR
flow cytometry
Co-IP

Software Mentioned

SPSS

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