Arabidopsis AAL-toxin-resistant mutant atr1 shows enhanced tolerance to programmed cell death induced by reactive oxygen species

Biochemical and Biophysical Research Communications
T S GechevJ Hille

Abstract

The fungal AAL-toxin triggers programmed cell death (PCD) through perturbations of sphingolipid metabolism in AAL-toxin-sensitive plants. While Arabidopsis is relatively insensitive to the toxin, the loh2 mutant exhibits increased susceptibility to AAL-toxin due to the knockout of a gene involved in sphingolipid metabolism. Genetic screening of mutagenized loh2 seeds resulted in the isolation of AAL-toxin-resistant mutant atr1.Atr1 displays a wild type phenotype when grown on soil but it develops less biomass than loh2 on media supplemented with 2% and 3% sucrose. Atr1 was also more tolerant to the reactive oxygen species-generating herbicides aminotriazole (AT) and paraquat. Microarray analyses of atr1 and loh2 under AT-treatment conditions that trigger cell death in loh2 and no visible damage in atr1 revealed genes specifically regulated in atr1 or loh2. In addition, most of the genes strongly downregulated in both mutants were related to cell wall extension and cell growth, consistent with the apparent and similar AT-induced cessation of growth in both mutants. This indicates that two different pathways, a first controlling growth inhibition and a second triggering cell death, are associated with AT-induced oxidative stress.

References

Jul 19, 2001·Nature·J L Dangl, J D Jones
Sep 13, 2001·Plant Physiology·R DesikanS J Neill
Oct 16, 2001·Cell Stress & Chaperones·B L LinM Delseny
May 23, 2002·Cellular and Molecular Life Sciences : CMLS·T GechevI Minkov
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eva VranováWim Van Camp
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Stefka D SpassievaJacques Hille
Nov 13, 2003·Current Opinion in Plant Biology·Yi LiSimon McQueen-Mason
Nov 13, 2003·Current Opinion in Plant Biology·Emma Pilling, Herman Höfte
Jun 18, 2004·The Plant Journal : for Cell and Molecular Biology·Steven VandenabeeleFrank Van Breusegem
Jan 6, 2005·The Journal of Cell Biology·Tsanko S Gechev, Jacques Hille
May 5, 2005·The New Phytologist·Patrick RomanoSheng Luan
Oct 17, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tsanko S GechevChristophe Laloi

❮ Previous
Next ❯

Citations

Oct 13, 2010·Protoplasma·Jens TilsnerLesley Torrance
Nov 22, 2011·Biological Trace Element Research·María Florencia IannoneMaría Patricia Benavides
Apr 20, 2012·Molecular Plant-microbe Interactions : MPMI·Keisuke MaseHirofumi Yoshioka
Aug 1, 2012·FEMS Microbiology Reviews·Takashi TsugeHiroshi Otani
Apr 2, 2011·Plant Signaling & Behavior·Daniel Da SilvaPatrice Thuleau
Jan 1, 2010·The Arabidopsis Book·Kristin Laluk, Tesfaye Mengiste
Jan 17, 2020·International Journal of Molecular Sciences·Mohammad Amin OmidbakhshfardTsanko S Gechev
Nov 17, 2020·Frontiers in Plant Science·Hong-Yun ZengNan Yao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis