Arabidopsis chlorophyll biosynthesis: an essential balance between the methylerythritol phosphate and tetrapyrrole pathways

The Plant Cell
Se KimJanet Braam

Abstract

Chlorophyll, essential for photosynthesis, is composed of a chlorin ring and a geranylgeranyl diphosphate (GGPP)-derived isoprenoid, which are generated by the tetrapyrrole and methylerythritol phosphate (MEP) biosynthesis pathways, respectively. Although a functional MEP pathway is essential for plant viability, the underlying basis of the requirement has been unclear. We hypothesized that MEP pathway inhibition is lethal because a reduction in GGPP availability results in a stoichiometric imbalance in tetrapyrrolic chlorophyll precursors, which can cause deadly photooxidative stress. Consistent with this hypothesis, lethality of MEP pathway inhibition in Arabidopsis thaliana by fosmidomycin (FSM) is light dependent, and toxicity of MEP pathway inhibition is reduced by genetic and chemical impairment of the tetrapyrrole pathway. In addition, FSM treatment causes a transient accumulation of chlorophyllide and transcripts associated with singlet oxygen-induced stress. Furthermore, exogenous provision of the phytol molecule reduces FSM toxicity when the phytol can be modified for chlorophyll incorporation. These data provide an explanation for FSM toxicity and thereby provide enhanced understanding of the mechanisms of FSM resist...Continue Reading

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Citations

May 17, 2015·Biochimica Et Biophysica Acta·Pawel BrzezowskiBernhard Grimm
Jan 7, 2016·Annual Review of Plant Biology·Kai Xun ChanBarry J Pogson
Feb 15, 2018·The Plant Journal : for Cell and Molecular Biology·Josephine HerbstBernhard Grimm
Apr 28, 2017·Molecular Ecology Resources·Tobias LortzingAnke Steppuhn
Jul 26, 2018·The Plant Journal : for Cell and Molecular Biology·Marcin JanowskiMarek Mutwil
Dec 24, 2018·Journal of Experimental Botany·Manuel Rodriguez-ConcepcionPablo Pulido
Apr 17, 2019·Journal of Experimental Botany·Vivek DograChanhong Kim
Sep 11, 2019·Journal of Experimental Botany·Matthew E BergmanMichael A Phillips
Jul 5, 2018·Frontiers in Plant Science·Dan ZhangMin Xu
May 18, 2021·Frontiers in Plant Science·Katalin Solymosi, Beata Mysliwa-Kurdziel

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