Arabidopsis cotyledon chloroplast biogenesis factor CYO1 uses glutathione as an electron donor and interacts with PSI (A1 and A2) and PSII (CP43 and CP47) subunits

Journal of Plant Physiology
Atsuko MuranakaHiroshi Shimada

Abstract

CYO1 is required for thylakoid biogenesis in cotyledons of Arabidopsis thaliana. To elucidate the enzymatic characteristics of CYO1, we analyzed the protein disulfide isomerase (PDI) activity of CYO1 using dieosin glutathione disulfide (Di-E-GSSG) as a substrate. The reductase activity of CYO1 increased as a function of Di-E-GSSG, with an apparent K(m) of 824nM and K(cat) of 0.53min(-1). PDI catalyzes dithiol/disulfide interchange reactions, and the cysteine residues in PDI proteins are very important. To analyze the significance of the cysteine residues for the PDI activity of CYO1, we estimated the kinetic parameters of point-mutated CYO1 proteins. C117S, C124S, C135S, and C156S had higher values for K(m) than did wild-type CYO1. C158S had a similar K(m) but a higher K(cat), and C138S and C161S had similar K(m) values but lower K(cat) values than did wild-type CYO1. These results suggested that the cysteine residues at positions 138 and 161 were important for PDI activity. Low PDI activity of CYO1 was observed when NADPH or NADH was used as an electron donor. However, PDI activity was observed with CYO1 and glutathione, suggesting that glutathione may serve as a reducing agent for CYO1 in vivo. Based on analysis with the spli...Continue Reading

References

Sep 29, 1995·The Journal of Biological Chemistry·A de Crouy-ChanelG Richarme
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May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock

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Citations

Jan 8, 2013·Antioxidants & Redox Signaling·Thomas Kieselbach
Nov 5, 2013·International Journal of Cell Biology·Yayoi Onda
Jan 17, 2015·International Journal of Molecular Sciences·Nitin A PatilMohammed Akhter Hossain
Sep 26, 2015·The Plant Journal : for Cell and Molecular Biology·Yubing LiDonna E Fernandez
Oct 9, 2014·Frontiers in Plant Science·Sazzad Karim, Henrik Aronsson
Dec 23, 2016·The Plant Journal : for Cell and Molecular Biology·Tamara Muñoz-NortesJosé Luis Micol
Nov 23, 2019·The Plant Journal : for Cell and Molecular Biology·Florian A BuschHiroshi Shimada
Aug 18, 2018·Photosynthesis Research·Emelie Lindquist, Henrik Aronsson

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