Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids.

Plant Molecular Biology
Leeann E ThorntonMichael M Neff


Cytochrome P450 monooxygenases (P450s) are a diverse family of proteins that have specialized roles in secondary metabolism and in normal cell development. Two P450s in particular, CYP734A1 and CYP72C1, have been identified as brassinosteroid-inactivating enzymes important for steroid-mediated signal transduction in Arabidopsis thaliana. Genetic analyses have demonstrated that these P450s modulate growth throughout plant development. While members of the CYP734A subfamily inactivate brassinosteroids through C-26 hydroxylation, the biochemical activity of CYP72C1 is unknown. Because CYP734A1 and CYP72C1 in Arabidopsis diverge more than brassinosteroid inactivating P450s in other plants, this study examines the structure and biochemistry of each enzyme. Three-dimensional models were generated to examine the substrate binding site structures and determine how they might affect the function of each P450. These models have indicated that the active site of CYP72C1 does not contain several conserved amino acids typically needed for substrate hydroxylation. Heterologous expression of these P450s followed by substrate binding analyses have indicated that CYP734A1 binds active brassinosteroids, brassinolide and castasterone, as well as ...Continue Reading


Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A Gegner, F W Dahlquist
Jun 5, 1987·Journal of Molecular Biology·T L PoulosA J Howard
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·W M Barnes
Aug 27, 1999·Archives of Biochemistry and Biophysics·S E Graham, J A Peterson
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M M NeffJ Chory
Feb 11, 2000·Vitamins and Hormones·G D SzklarzM D Paulsen
Oct 29, 2002·The Journal of Biological Chemistry·Jason K YanoThomas L Poulos
Nov 19, 2002·The Journal of Biological Chemistry·David LeysAndrew W Munro
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Hideo NakashitaShigeo Yoshida
Jul 16, 2003·Nature·Pamela A WilliamsHarren Jhoti
Aug 23, 2003·The Journal of Biological Chemistry·Shingo NaganoThomas L Poulos
Sep 25, 2003·Annual Review of Plant Biology·Shozo Fujioka, Takao Yokota
Sep 25, 2003·Annual Review of Plant Biology·Mary A Schuler, Daniele Werck-Reichhart
Dec 17, 2003·The Journal of Biological Chemistry·Guillaume A SchochEric F Johnson
Apr 23, 2004·Pharmacogenomics·David F V Lewis
Jun 29, 2004·Journal of Inorganic Biochemistry·Rebecca C Wade Sudarko
Jul 20, 2004·The Journal of Biological Chemistry·Jason K YanoEric F Johnson
Nov 9, 2004·Structure·Larissa M PodustMichael R Waterman
Dec 8, 2004·Archives of Biochemistry and Biophysics·Carol A KempMichael J Sutcliffe
Feb 4, 2005·Journal of Experimental Botany·Masanobu NakamuraAkira Nagatani
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Naoki TakahashiMinami Matsui
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Edward M TurkMichael M Neff
Sep 15, 2005·Biochemical and Biophysical Research Communications·Eric F Johnson, C David Stout
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brigitte PoppenbergerDianna Bowles
Jun 17, 2006·Protein Engineering, Design & Selection : PEDS·Jerome BaudryMary A Schuler
Jul 29, 2006·Phytochemistry·Toshiyuki OhnishiMasaharu Mizutani
Apr 13, 2007·Proteins·Sangeewa G RupasingheMary A Schuler
Oct 13, 2009·Phytochemistry·Toshiyuki OhnishiMasaharu Mizutani
Mar 3, 2010·Annual Review of Plant Biology·Tae-Wuk Kim, Zhi-Yong Wang
Jan 1, 2002·The Arabidopsis Book·Danièle Werck-ReichhartSuzanne Paquette
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus

❮ Previous
Next ❯


Jul 8, 2011·Planta·Leeann E ThorntonMichael M Neff
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Björn Hamberger, Søren Bak
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·David Nelson, Danièle Werck-Reichhart
May 11, 2011·Journal of Integrative Plant Biology·Huaxun YeYanhai Yin
Oct 2, 2012·The Plant Journal : for Cell and Molecular Biology·Sunhwa ChoiIldoo Hwang
Sep 12, 2012·Journal of Integrative Plant Biology·Baolin Zhao, Jia Li
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Lijuan ChaiHuiqin Ma
Feb 5, 2014·Plant Signaling & Behavior·Kulbir Singh Sandhu, Michael M Neff
Feb 4, 2012·The Arabidopsis Book·Søren BakDanièle Werck-Reichhart
Feb 4, 2012·The Arabidopsis Book·Steven D Clouse
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wilfried RozhonBrigitte Poppenberger
Nov 3, 2020·Frontiers in Plant Science·Zhuoyun Wei, Jia Li
Feb 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pauline van HaperenBen Vosman

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.