Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids.

Plant Molecular Biology
Leeann E ThorntonMichael M Neff

Abstract

Cytochrome P450 monooxygenases (P450s) are a diverse family of proteins that have specialized roles in secondary metabolism and in normal cell development. Two P450s in particular, CYP734A1 and CYP72C1, have been identified as brassinosteroid-inactivating enzymes important for steroid-mediated signal transduction in Arabidopsis thaliana. Genetic analyses have demonstrated that these P450s modulate growth throughout plant development. While members of the CYP734A subfamily inactivate brassinosteroids through C-26 hydroxylation, the biochemical activity of CYP72C1 is unknown. Because CYP734A1 and CYP72C1 in Arabidopsis diverge more than brassinosteroid inactivating P450s in other plants, this study examines the structure and biochemistry of each enzyme. Three-dimensional models were generated to examine the substrate binding site structures and determine how they might affect the function of each P450. These models have indicated that the active site of CYP72C1 does not contain several conserved amino acids typically needed for substrate hydroxylation. Heterologous expression of these P450s followed by substrate binding analyses have indicated that CYP734A1 binds active brassinosteroids, brassinolide and castasterone, as well as ...Continue Reading

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Citations

Jul 8, 2011·Planta·Leeann E ThorntonMichael M Neff
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Björn Hamberger, Søren Bak
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·David Nelson, Danièle Werck-Reichhart
May 11, 2011·Journal of Integrative Plant Biology·Huaxun YeYanhai Yin
Oct 2, 2012·The Plant Journal : for Cell and Molecular Biology·Sunhwa ChoiIldoo Hwang
Sep 12, 2012·Journal of Integrative Plant Biology·Baolin Zhao, Jia Li
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Lijuan ChaiHuiqin Ma
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Feb 4, 2012·The Arabidopsis Book·Søren BakDanièle Werck-Reichhart
Feb 4, 2012·The Arabidopsis Book·Steven D Clouse
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wilfried RozhonBrigitte Poppenberger
Nov 3, 2020·Frontiers in Plant Science·Zhuoyun Wei, Jia Li
Feb 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pauline van HaperenBen Vosman

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