Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.

Scientific Reports
Yukihiro NagashimaNozomu Koizumi

Abstract

IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes whose induction was reduced in ire1a/ire1b largely overlapped those in the bzip60 mutant. We observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b. We further demonstrated that bZIP60 mRNA is spliced by ER stress, removing 23 ribonucleotides and therefore causing a frameshift that replaces the C-terminal region of bZIP60 including the transmembrane domain (TMD) with a shorter region without a TMD. This splicing was detected in ire1a and ire1b single mutants, but not in the ire1a/ire1b double mutant. We conclude that IRE1A and IRE1B catalyse unconventional splicing of bZIP60 mRNA to produce the active transcription factor.

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Citations

Feb 2, 2013·Plant Molecular Biology·Kyoko KudoFumio Takaiwa
Jul 25, 2013·Trends in Cell Biology·Yani Chen, Federica Brandizzi
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kei-ichiro MishibaNozomu Koizumi
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yan DengStephen H Howell
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Datasets Mentioned

BETA
E-MEXP-3186

Methods Mentioned

BETA
glycosylation
PCR

Software Mentioned

R
ClustalW
GeneChip Operating Software
BLASTn
Affymetrix
MeV
MEGA4
CentroidFold

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