Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K(+)/H(+) antiporter with a chloroplast transit peptide

Biochimica Et Biophysica Acta
Maria Nieves Aranda-SiciliaKees Venema

Abstract

KEA genes encode putative K(+) efflux antiporters that are predominantly found in algae and plants but are rare in metazoa; however, nothing is known about their functions in eukaryotic cells. Plant KEA proteins show homology to bacterial K(+) efflux (Kef) transporters, though two members in the Arabidopsis thaliana family, AtKEA1 and AtKEA2, have acquired an extra hydrophilic domain of over 500 residues at the amino terminus. We show that AtKEA2 is highly expressed in leaves, stems and flowers, but not in roots, and that an N-terminal peptide of the protein is targeted to chloroplasts in Arabidopsis cotyledons. The full-length AtKEA2 protein was inactive when expressed in yeast; however, a truncated AtKEA2 protein (AtsKEA2) lacking the N-terminal domain complemented disruption of the Na(+)(K(+))/H(+) antiporter Nhx1p to confer hygromycin resistance and tolerance to Na(+) or K(+) stress. To test transport activity, purified truncated AtKEA2 was reconstituted in proteoliposomes containing the fluorescent probe pyranine. Monovalent cations reduced an imposed pH gradient (acid inside) indicating AtsKEA2 mediated cation/H(+) exchange with preference for K(+)=Cs(+)>Li(+)>Na(+). When a conserved Asp(721) in transmembrane helix 6 that...Continue Reading

References

May 1, 1992·The Plant Journal : for Cell and Molecular Biology·M MinetF Lacroute
May 25, 1999·Protein Science : a Publication of the Protein Society·O EmanuelssonG von Heijne
Aug 14, 2001·Plant Physiology·P MäserM L Guerinot
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·X WangJ S Peters
Nov 15, 2001·The Journal of Biological Chemistry·Kees VenemaJuan Pedro Donaire
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Jul 26, 2002·Analytical Biochemistry·Madhu V Singh, P Anthony Weil
Apr 16, 2003·The Journal of Biological Chemistry·Kees VenemaJuan Pedro Donaire
Aug 27, 2003·Biochemistry·Ian R BoothSamantha Miller
Jun 19, 2004·Molecular Membrane Biology·Abraham B ChangMilton H Saier
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Françoise CellierFrancine Casse
Jan 7, 2005·Molecular Biology of the Cell·Christopher L BrettRajini Rao
Jan 12, 2005·American Journal of Physiology. Cell Physiology·Christopher L BrettRajini Rao
May 28, 2005·Science·Daniel O DaleyGunnar von Heijne
Mar 4, 2006·Journal of Experimental Botany·D HallJ Pritchard
Sep 1, 1988·Plant Physiology·A S Gupta, G A Berkowitz
Mar 6, 2007·Plant Physiology·Senthilkumar PadmanabanHeven Sze
Aug 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Makoto FujisawaTerry A Krulwich
Aug 11, 2007·Science·Isaiah T ArkinDavid E Shaw
Feb 23, 2008·Plant Molecular Biology·Toshifumi NagataShoshi Kikuchi
Aug 5, 2008·Plant Physiology·Jian ZhaoKendal D Hirschi
Nov 17, 2009·The Plant Journal : for Cell and Molecular Biology·Eduardo O LeidiJosé M Pardo
Aug 17, 2010·The Journal of Biological Chemistry·Olivier CagnacKees Venema
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tarmo P RoosildIan R Booth
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Francisco J QuinteroJose M Pardo

❮ Previous
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Citations

Jul 10, 2013·Cellular and Molecular Life Sciences : CMLS·Bernard E PfeilCornelia Spetea
May 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hans-Henning KunzJulian I Schroeder
Dec 3, 2014·Nature Communications·Ute ArmbrusterMartin C Jonikas
Mar 15, 2016·Frontiers in Plant Science·Ricarda HöhnerKees Venema
Jun 22, 2013·Physiologia Plantarum·Yunliu ZengXiuxin Deng
Jan 8, 2014·Journal of Integrative Plant Biology·Eri Adams, Ryoung Shin
Apr 5, 2015·Journal of Plant Physiology·Edgar Pinto, Isabel M P L V O Ferreira
Mar 19, 2014·Journal of Plant Physiology·Noa WigodaNava Moran
May 9, 2014·Journal of Plant Physiology·Izhar Ahmad, Frans J M Maathuis
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Aaron B StephanJulian I Schroeder
Oct 27, 2016·The Plant Journal : for Cell and Molecular Biology·Caijuan WangToshiharu Shikanai
Feb 20, 2018·Journal of Dairy Science·Maofeng ZhangYingwang Ye
Jan 14, 2018·Scientific Reports·Tapan Kumar MondalTilak Raj Sharma
Jun 19, 2014·Molecules and Cells·Ryoung Shin
Jul 13, 2019·Scientific Reports·Masaru TsujiiNobuyuki Uozumi
Aug 10, 2020·Physiologia Plantarum·Insha AminParvaiz Ahmad
Mar 25, 2017·Journal of Experimental Botany·Ildikò Szabò, Cornelia Spetea
May 5, 2017·Journal of Experimental Botany·Jayakumar BoseStephen D Tyerman
Oct 13, 2018·Nature Communications·Gal MasratiNir Ben-Tal
Jul 2, 2020·International Journal of Molecular Sciences·Masaru TsujiiNobuyuki Uozumi
Apr 10, 2019·Molecular & Cellular Proteomics : MCP·Imen BouchnakNorbert Rolland
Oct 31, 2020·Frontiers in Plant Science·Stanislav V IsayenkovSergey Shabala
Oct 29, 2020·The New Phytologist·María Nieves Aranda SiciliaKees Venema
Jul 1, 2019·Molecular & Cellular Proteomics : MCP·Imen BouchnakNorbert Rolland
Jul 9, 2021·The Plant Cell·Rachael Ann DeTarHans-Henning Kunz

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