Abstract
The plant hormone, abscisic acid (ABA), is a main signal transducer that confers abiotic stress tolerance to plants. Although the pathway of ABA production and the genes catalyzing its biosynthesis are largely defined, the regulatory mechanism of ABA biosynthesis in response to abiotic stress remains much unknown. In this study, to identify upstream genes regulating ABA biosynthesis involved in abiotic stress signal transduction, Arabidopsis thaliana mutants with altered promoter activity of 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), a key gene in ABA biosynthesis, were identified and characterized. Among selected mutants, lenc1 (for low expression of NCED3 1) after dehydration treatment had lower AtNCED3 promoter activity compared with wild type. lenc1 mutation is recessive and is located on chromosome 4. Expression analysis of AtNCED3 and quantification of ABA levels showed that both the AtNCED3 transcripts and the endogenous ABA in lenc1 were less abundant than in wild type under dehydration treatments. The lenc1 was hypersensitive to methyl viologen (MV), LiCl, NaCl and high light. The aerial part of lenc1 lost water faster than wild type possibly due to a larger stomata opening. Our results suggest LENC1 might act as a p...Continue Reading
References
Dec 1, 1992·Plant Molecular Biology·V Lång, E T Palva
Aug 15, 1991·Gene·M C ShihH M Goodman
Jan 1, 1993·Molecular & General Genetics : MGG·K Yamaguchi-Shinozaki, K Shinozaki
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KovtunJ Sheen
Sep 5, 2001·The Plant Journal : for Cell and Molecular Biology·S IuchiK Shinozaki
Sep 11, 2001·The Plant Cell·L XiongJ K Zhu
Feb 14, 2002·Plant Physiology·Xiaoqiong Qin, Jan A D Zeevaart
Jul 11, 2002·Science's STKE : Signal Transduction Knowledge Environment·Viswanathan ChinnusamyJian-Kang Zhu
Apr 15, 2003·Plant Physiology·Steven H SchwartzJan A D Zeevaart
Sep 13, 2003·Plant Physiology·Liming Xiong, Jian-Kang Zhu
Jun 23, 2004·The Plant Cell·Girdhar K PandeySheng Luan
Oct 7, 2004·Plant Physiology·Bruno RuggieroAlbino Maggio
Jun 6, 2006·The Plant Cell·Byeong-ha LeeJian-Kang Zhu
Jun 24, 2006·The Plant Journal : for Cell and Molecular Biology·Jae-Heung KoKyung-Hwan Han
Jul 28, 2006·Biochemical and Biophysical Research Communications·Xiao-Rong Wan, Ling Li
Aug 26, 2006·Plant, Cell & Environment·José María BarreroJosé Luis Micol
Aug 6, 2008·Biochemical and Biophysical Research Communications·Hee-Yeon ParkYong-Hwan Moon
Oct 28, 2008·Biotechnology Advances·M Ashraf
Nov 19, 2008·Plant Methods·Nick S WooBarry J Pogson
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Kaoru UranoKazuo Shinozaki
Mar 17, 2009·Biochemical and Biophysical Research Communications·Hee-Yeon ParkYong-Hwan Moon
Oct 10, 2009·Plant Signaling & Behavior·Yu'e Zhang, Yongbiao Xue
Citations
Jan 30, 2014·PloS One·Yan-Zhuo Yang, Bao-Cai Tan
Apr 29, 2016·BMC Plant Biology·Sara I ZandalinasVicent Arbona
Oct 23, 2016·Planta·Hye-Yeon SeokYong-Hwan Moon
Aug 30, 2020·The Plant Journal : for Cell and Molecular Biology·Liem T BuiBeatrice Giuntoli
Jul 23, 2021·Plant Physiology and Biochemistry : PPB·Meng LiHongyan Qi