Arabidopsis molybdenum cofactor sulfurase ABA3 contributes to anthocyanin accumulation and oxidative stress tolerance in ABA-dependent and independent ways

Scientific Reports
Shunsuke WatanabeMitsunori Seo

Abstract

Arabidopsis ABA3 is an enzyme involved in the synthesis of the sulfurated form of the molybdenum (Mo) cofactor (MoCo), which is required for the enzymatic activity of so-called Mo enzymes such as aldehyde oxidase (AO) and xanthine dehydrogenase (XDH). It has been reported that AO and XDH are essential for the biosynthesis of the bioactive compounds, ABA and allantoin, respectively. However, aba3 mutants often exhibit pleiotropic phenotypes that are not explained by defects in ABA and/or allantoin biosynthesis, leading us to hypothesize that ABA3 regulates additional metabolic pathways. To reveal the currently unidentified functions of ABA3 we compared transcriptome and metabolome of the Arabidopsis aba3 mutant with those of wild type and a typical ABA-deficient mutant aba2. We found that endogenous levels of anthocyanins, members of the flavonoid group, were significantly lower in the aba3 mutant than in the wild type or the aba2 mutant under oxidative stress. In contrast, mutants defective in the AO and XDH holoenzymes accumulated significantly higher levels of anthocyanins when compared with aba3 mutant under the same conditions. Our findings shed light on a key role of ABA3 in the ABA- and allantoin-independent accumulation ...Continue Reading

References

Oct 16, 2019·Frontiers in Plant Science·Abrar FelembanSalim Al-Babili
Nov 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shunsuke WatanabeMitsunori Seo

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Related Concepts

Biological Adaptation to Stress
Enzymes, antithrombotic
Arabidopsis
3-hydroxyflavone
Sulfur
Mutant Proteins
Oxidative Stress
Oxidative Stress Analysis
ABA3 protein, Arabidopsis
Molybdenum cofactor

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