Arabidopsis phot1 and phot2 phosphorylate BLUS1 kinase with different efficiencies in stomatal opening

Journal of Plant Research
Atsushi Takemiya, Ken-ichiro Shimazaki

Abstract

In Arabidopsis thaliana, phototropins (phot1 and phot2), light-activated receptor kinases, redundantly regulate various photoresponses such as phototropism, chloroplast photorelocation movement, stomatal opening, and leaf flattening. However, it is still unclear how phot1 and phot2 signals are integrated into a common target and regulate physiological responses. In the present study, we provide evidence that phot1 and phot2 phosphorylate BLUE LIGHT SIGNALING1 (BLUS1) kinase as a common substrate in stomatal opening. Biochemical analysis revealed that the recombinant phot2 protein directly phosphorylated BLUS1 in vitro in a blue light-dependent manner, as reported for phot1. BLUS1 phosphorylation was observed in both phot1 and phot2 mutants, and phot2 mutant exhibited higher phosphorylation of BLUS1 than did phot1 mutant. Transgenic plants expressing phot1-GFP (P1G) and phot2-GFP (P2G) at a similar level under the PHOT2 promoter demonstrated that P1G initiated higher phosphorylation of BLUS1 than P2G, suggesting that phot1 phosphorylates BLUS1 more efficiently. Similarly, P1G mediated a higher activation of the plasma membrane H(+)-ATPase and stomatal opening than P2G, indicating that the phosphorylation status of BLUS1 is a key...Continue Reading

References

Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J M ChristieW R Briggs
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SakaiK Okada
Jul 10, 2001·The Journal of Biological Chemistry·T E SwartzR A Bogomolni
Dec 12, 2001·Nature·T KinoshitaK Shimazaki
Aug 13, 2002·The Plant Cell·Koji Sakamoto, Winslow R Briggs
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·John M ChristieWinslow R Briggs
Mar 26, 2003·Plant Molecular Biology·Kevin M Folta, Lon S Kaufman
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner
Mar 8, 2005·The Plant Cell·Atsushi TakemiyaKen-Ichiro Shimazaki
Sep 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Daisuke Matsuoka, Satoru Tokutomi
Sep 20, 2005·Journal of Molecular Evolution·Patricia Lariguet, Christophe Dunand
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Sam-Geun KongAkira Nagatani
Jun 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Patricia LariguetChristian Fankhauser
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Atsushi TakemiyaKen-Ichiro Shimazaki
Oct 28, 2006·Annual Review of Plant Biology·John M Christie
Dec 14, 2006·The Journal of Biological Chemistry·Matthew A JonesJohn M Christie
Jan 11, 2007·Annual Review of Plant Biology·Ken-ichiro ShimazakiToshinori Kinoshita
Jul 27, 2007·Plant & Cell Physiology·Kosei IwabuchiShingo Takagi
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·Sam-Geun KongAkira Nagatani
Nov 21, 2007·Plant Physiology·Hernán E BoccalandroJorge J Casal
Apr 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shin-Ichiro InoueKen-Ichiro Shimazaki
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Yusuke AiharaAkira Nagatani
Oct 22, 2008·Current Opinion in Plant Biology·Emilie Demarsy, Christian Fankhauser
Jan 1, 2008·Molecular Plant·Shin-ichiro InoueKen-ichiro Shimazaki
Oct 6, 2010·Current Opinion in Plant Biology·Shin-ichiro InoueKen-ichiro Shimazaki
Jul 14, 2011·International Review of Cell and Molecular Biology·Toshinori Kinoshita, Yuki Hayashi
Mar 13, 2012·Journal of Molecular Biology·Josiah P ZaynerTobin R Sosnick
Jun 29, 2012·Plant & Cell Physiology·Akiko HaradaKen-ichiro Shimazaki
Jul 12, 2012·The EMBO Journal·Emilie DemarsyChristian Fankhauser
Feb 15, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Peter L FreddolinoKlaus Schulten
Jul 3, 2013·Nature Communications·Atsushi TakemiyaKen-ichiro Shimazaki
Aug 13, 2013·The Journal of Physical Chemistry. B·Kimitoshi TakedaMasahide Terazima

❮ Previous
Next ❯

Citations

Feb 11, 2016·Journal of Plant Research·Sam-Geun Kong, Koji Okajima
Feb 25, 2018·The Journal of Biological Chemistry·Jonathan SchnabelJohn M Christie
Nov 5, 2019·Frontiers in Genetics·Mohammad BablaZhong-Hua Chen
Dec 25, 2019·Journal of Experimental Botany·Jack S A MatthewsTracy Lawson
Jun 21, 2018·Nature Communications·Paolo SchumacherChristian Fankhauser
Mar 31, 2020·Photosynthesis Research·Arkajo Majumdar, Rup Kumar Kar
May 7, 2020·Frontiers in Plant Science·Yuanyuan Wang, Zhong-Hua Chen
Aug 28, 2020·Frontiers in Plant Science·Lixia Pan, Ive De Smet
Dec 22, 2020·Frontiers in Plant Science·Juan YangHongbin Wei
Aug 16, 2016·Current Opinion in Plant Biology·Sarah M Assmann, Timothy Jegla
Jun 9, 2021·The New Phytologist·Silvere Vialet-ChabrandTracy Lawson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.