Arabidopsis Protein Kinase D6PKL3 Is Involved in the Formation of Distinct Plasma Membrane Aperture Domains on the Pollen Surface

The Plant Cell
Byung Ha LeeAnna Dobritsa

Abstract

Certain regions on the surfaces of developing pollen grains exhibit very limited deposition of pollen wall exine. These regions give rise to pollen apertures, which are highly diverse in their patterns and specific for individual species. Arabidopsis thaliana pollen develops three equidistant longitudinal apertures. The precision of aperture formation suggests that, to create them, pollen employs robust mechanisms that generate distinct cellular domains. To identify players involved in this mechanism, we screened natural Arabidopsis accessions and discovered one accession, Martuba, whose apertures form abnormally due to the disruption of the protein kinase D6PKL3. During pollen development, D6PKL3 accumulates at the three plasma membrane domains underlying future aperture sites. Both D6PKL3 localization and aperture formation require kinase activity. Proper D6PKL3 localization is also dependent on a polybasic motif for phosphoinositide interactions, and we identified two phosphoinositides that are specifically enriched at the future aperture sites. The other known aperture factor, INAPERTURATE POLLEN1, fails to aggregate at the aperture sites in d6pkl3 mutants, changes its localization when D6PKL3 is mislocalized, and, in turn,...Continue Reading

Citations

May 23, 2020·Annual Review of Plant Biology·Lise C Noack, Yvon Jaillais
Sep 30, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Asja Radja
Aug 7, 2019·International Journal of Molecular Sciences·Zdeňka KubátováIvan Kulich
Apr 15, 2020·Nature Plants·Yuan Zhou, Anna A Dobritsa
Mar 12, 2021·Journal of Plant Physiology·Xiaofeng MaGenfa Zhang
Sep 16, 2019·Plant Science : an International Journal of Experimental Plant Biology·Yuan Zhou, Anna A Dobritsa
Apr 27, 2021·Annual Review of Plant Biology·Said Hafidh, David Honys
Jun 30, 2021·Nature Plants·Byung Ha LeeAnna A Dobritsa
Jul 27, 2021·Frontiers in Plant Science·Ismael Mazuecos-AguileraVíctor N Suárez-Santiago
Sep 16, 2021·Journal of Experimental Botany·Małgorzata LichockaJacek Hennig

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