Sep 20, 2005

Arabidopsis RAD51C gene is important for homologous recombination in meiosis and mitosis

Plant Physiology
Kiyomi AbeSeiichi Toki

Abstract

Rad51 is a homolog of the bacterial RecA recombinase, and a key factor in homologous recombination in eukaryotes. Rad51 paralogs have been identified from yeast to vertebrates. Rad51 paralogs are thought to play an important role in the assembly or stabilization of Rad51 that promotes homologous pairing and strand exchange reactions. We previously characterized two RAD51 paralogous genes in Arabidopsis (Arabidopsis thaliana) named AtRAD51C and AtXRCC3, which are homologs of human RAD51C and XRCC3, respectively, and described the interaction of their products in a yeast two-hybrid system. Recent studies showed the involvement of AtXrcc3 in DNA repair and functional role in meiosis. To determine the role of RAD51C in meiotic and mitotic recombination in higher plants, we characterized a T-DNA insertion mutant of AtRAD51C. Although the atrad51C mutant grew normally during vegetative developmental stage, the mutant produced aborted siliques, and their anthers did not contain mature pollen grains. Crossing of the mutant with wild-type plants showed defective male and female gametogeneses as evidenced by lack of seed production. Furthermore, meiosis was severely disturbed in the mutant. The atrad51C mutant also showed increased sensi...Continue Reading

  • References56
  • Citations54

Mentioned in this Paper

Vertebrates
Yeast Two-Hybrid System Techniques
Genes, Plant
XRCC3 gene
DNA Repair
Arabidopsis
RAD51C protein, human
Recombination, Genetic
XRCC3 protein, human
Recombinase

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