Arabidopsis response regulator 22 inhibits cytokinin-regulated gene transcription in vivo

Protoplasma
Niklas WallmerothVirtudes Mira-Rodado

Abstract

Cytokinin signaling in Arabidopsis is carried out by a two-component system (TCS) multi-step phosphorelay mechanism that involves three different protein families: histidine kinases (AHKs), phosphotransfer proteins (AHPs), and response regulators (ARRs) that are in turn, subdivided into A-, B- and C-type ARRs depending on their function and structure. Upon cytokinin perception, AHK proteins autophosphorylate; this phosphate is then transferred from the AHKs to the AHPs to finally reach the ARRs. When B-type ARRs are activated by phosphorylation, they function as transcription factors that regulate the expression of cytokinin-dependent genes such as the A-type ARRs, among many others. In cytokinin signaling, while A- and B-type ARR function is well understood, it is still unclear if C-type ARRs (ARR22 and ARR24) play a role in this mechanism. Here, we describe a novel method suitable to study TCS activity natively as an in vivo system. We also show that ARR22 inhibits gene transcription of an A-type ARR upon cytokinin treatment in vivo. Consequently, we propose that ARR22, by acting as a phosphatase on specific AHPs, disrupts the TCS phosphorelay and prevents B-type ARR phosphorylation, and thus their activation as transcription...Continue Reading

References

May 3, 2000·The Plant Journal : for Cell and Molecular Biology·M Sprenger-Haussels, B Weisshaar
Feb 28, 2009·Current Opinion in Microbiology·Urs Jenal, Michael Y Galperin
Aug 13, 2010·Journal of the Royal Society, Interface·Attila Csikász-NagyOrkun S Soyer
Jan 24, 2012·Journal of Theoretical Biology·Michael KnudsenCarsten Wiuf
Jan 1, 2008·The Arabidopsis Book·G Eric SchallerShin-Han Shiu
Jul 11, 2012·The Plant Journal : for Cell and Molecular Biology·Manikandan VeerabaguVirtudes Mira-Rodado
Jan 28, 2014·The Arabidopsis Book·Joseph J Kieber, G Eric Schaller

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