Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome

Plants
Veronika BerkováMartin Cerny

Abstract

Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed Arabidopsis thaliana response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously rep...Continue Reading

References

May 1, 1995·Plant Physiology·J. Deikman, P. E. Hammer
Jun 25, 2005·Science·Motoyuki AshikariMakoto Matsuoka
Jun 3, 2008·Journal of Experimental Botany·Tomás WernerThomas Schmülling
Jun 25, 2008·Journal of Experimental Botany·Michel Edmond GhanemFrancisco Pérez-Alfocea
Sep 27, 2008·Bioorganic & Medicinal Chemistry·Marek ZatloukalMiroslav Strnad
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kamil RuzickaEva Benková
Feb 16, 2011·Journal of Experimental Botany·Ivo FrébortPetr Galuszka
Mar 11, 2011·Nature Biotechnology·Aleksandra SkiryczDirk Inzé
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yue YangKatherine W Osteryoung
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jan 15, 2013·Journal of Experimental Botany·Ingela FridborgSarosh Bejai
Apr 19, 2013·Molecular Plant-microbe Interactions : MPMI·Michael ReuscheThomas Teichmann
Jan 13, 2015·Plant Science : an International Journal of Experimental Plant Biology·Jana DobráRadomíra Vanková
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Steffen ReinbotheChristiane Reinbothe
Apr 26, 2015·Journal of Experimental Botany·Paul J Zwack, Aaron M Rashotte
Jan 1, 2016·Bioorganic & Medicinal Chemistry·Radoslav KoprnaLukáš Spíchal
Jan 2, 2016·Biochimica Et Biophysica Acta·Martin ČernýBřetislav Brzobohatý
Apr 5, 2016·Journal of Plant Physiology·Jyoti DalalHeike W Sederoff
Nov 20, 2016·Methods in Molecular Biology·Chloé BéziatElena Feraru
Dec 3, 2016·Nucleic Acids Research·Cornelia M HooperA Harvey Millar
Aug 22, 2018·International Journal of Molecular Sciences·Jaroslav PavlůMartin Černý
Aug 25, 2018·Scientific Reports·Hendrik NolteMarcus Krüger
Nov 30, 2018·Plant, Cell & Environment·Anne CortlevenThomas Schmülling
Aug 20, 2019·Protein Science : a Publication of the Protein Society·Minoru Kanehisa, Yoko Sato
Dec 29, 2019·Bioinformatics·Steven Xijin GeRunan Yao
Mar 5, 2020·The New Phytologist·Sumudu S KarunadasaJan A Smalle
Mar 20, 2020·Nature·Julia MergnerBernhard Kuster
May 8, 2020·PloS One·H Tucker HallmarkAaron M Rashotte
Jul 17, 2020·Frontiers in Plant Science·Federico Martinez-SeidelJoachim Kopka
Dec 1, 2020·Frontiers in Plant Science·Miroslav BerkaMartin Černý

❮ Previous
Next ❯

Citations

Dec 17, 2020·International Journal of Molecular Sciences·Miroslav BerkaMartin Černý
Mar 27, 2021·Expert Review of Proteomics·J V Jorrin Novo

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
transgenic

Software Mentioned

Rapid Miner
ImageJ
Proteome Discoverer
Minora
Instant Clue
INCYDE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
T Kakimoto
The Plant Cell
Claire E Hutchison, Joseph J Kieber
Current Opinion in Plant Biology
T Kakimoto
© 2021 Meta ULC. All rights reserved