Arabidopsis RNASE THREE LIKE2 Modulates the Expression of Protein-Coding Genes via 24-Nucleotide Small Interfering RNA-Directed DNA Methylation

The Plant Cell
Emilie Elvira-MatelotHervé Vaucheret

Abstract

RNaseIII enzymes catalyze the cleavage of double-stranded RNA (dsRNA) and have diverse functions in RNA maturation. Arabidopsis thaliana RNASE THREE LIKE2 (RTL2), which carries one RNaseIII and two dsRNA binding (DRB) domains, is a unique Arabidopsis RNaseIII enzyme resembling the budding yeast small interfering RNA (siRNA)-producing Dcr1 enzyme. Here, we show that RTL2 modulates the production of a subset of small RNAs and that this activity depends on both its RNaseIII and DRB domains. However, the mode of action of RTL2 differs from that of Dcr1. Whereas Dcr1 directly cleaves dsRNAs into 23-nucleotide siRNAs, RTL2 likely cleaves dsRNAs into longer molecules, which are subsequently processed into small RNAs by the DICER-LIKE enzymes. Depending on the dsRNA considered, RTL2-mediated maturation either improves (RTL2-dependent loci) or reduces (RTL2-sensitive loci) the production of small RNAs. Because the vast majority of RTL2-regulated loci correspond to transposons and intergenic regions producing 24-nucleotide siRNAs that guide DNA methylation, RTL2 depletion modifies DNA methylation in these regions. Nevertheless, 13% of RTL2-regulated loci correspond to protein-coding genes. We show that changes in 24-nucleotide siRNA leve...Continue Reading

Citations

Jul 1, 2017·RNA Biology·Thomas MontavonPatrice Dunoyer
Sep 1, 2018·Plant & Cell Physiology·Chin Hong Lee, Bernard J Carroll
Jun 2, 2016·Nature Plants·Zhaobo Lang, Zhizhong Gong
Feb 25, 2017·RNA·Marie-Aude TschoppNathan Pumplin
Nov 16, 2017·Molecular Plant-microbe Interactions : MPMI·Nachelli Malpica-LópezMikhail M Pooggin
Nov 26, 2016·Journal of Plant Research·Akihito Fukudome, Toshiyuki Fukuhara
Feb 17, 2017·The Plant Journal : for Cell and Molecular Biology·Nicolas ButelTaline Elmayan
Jan 1, 2017·National Science Review·Huiming Zhang, Jian-Kang Zhu
Mar 25, 2020·International Journal of Molecular Sciences·Anran XuanDeqiang Zhang
May 11, 2021·Frontiers in Molecular Biosciences·Jiali YuMargaret E Staton
Dec 18, 2021·The Plant Journal : for Cell and Molecular Biology·Liangsheng WangTatjana Kleine

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