Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance

Botanical Studies
Li-Min HuangJei-Fu Shaw

Abstract

SFARs (seed fatty acid reducers) belonging to the GDSL lipases/esterases family have been reported to reduce fatty acid storage and composition in mature Arabidopsis seeds. GDSL lipases/esterases are hydrolytic enzymes that possess multifunctional properties, such as broad substrate specificity, regiospecificity, and stereoselectivity. Studies on the physiological functions and biochemical characteristics of GDSL lipases/esterases in plants are limited, so it is important to elucidate the molecular functions of GDSL-type genes. We found that SFAR4 (At3g48460), a fatty acid reducer belonging to the Arabidopsis GDSL lipases/esterases family, was intensely expressed in embryo protrusion, early seedlings, and pollen. The characterization of recombinant SFAR4 protein indicated that it has short-length p-nitrophenyl esterase activity. In addition, SFAR4 enhanced the expression of genes involved in fatty acid metabolism during seed germination and seedling development. SFAR4 elevated the expression of COMATOSE, which transports fatty acids into peroxisomes, and of LACS6 and LACS7, which deliver long-chain acetyl-CoA for β-oxidation. Furthermore, SFAR4 increased the transcription of PED1 and PNC1, which function in importing peroxisoma...Continue Reading

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Citations

Aug 26, 2017·Plant Molecular Biology·Chia-Ping LaiJei-Fu Shaw
Jun 7, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Steve LarsonKevin Jensen
Feb 19, 2020·Journal of Integrative Plant Biology·Huihui ZhangXiaoyan Tang
Apr 22, 2020·The Plant Journal : for Cell and Molecular Biology·Bo YuSheng Teng
Mar 7, 2020·Horticulture Research·Luciel Dos Santos FernandesStefan Royaert
Aug 24, 2019·Progress in Lipid Research·Kun WangChristoph Benning
May 13, 2019·Progress in Lipid Research·Kun WangChristoph Benning
Mar 2, 2021·Horticulture Research·Luciel Dos Santos FernandesStefan Royaert
May 16, 2017·Journal of Proteome Research·Dandan WangQingyou Xia

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Datasets Mentioned

BETA
SMD168H

Methods Mentioned

BETA
PCR
transgenic
glycosylation

Software Mentioned

Genevestigator
MEGA
Image J
SignalP
Multiple Em for Motif Elicitation ( MEME )
BLAST
MEME
ClustalW

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