Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress.

Annals of Botany
Natasha D SpadaforaDennis Francis

Abstract

In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana;CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested. Methods arath;cdc25 T-DNA insertion lines, Arath;CDC25 over-expressing lines and wild type were challenged with hydroxyurea (HU) and zeocin, substances that stall DNA replication and damage DNA, respectively, together with an abiotic stressor, NaCl. A molecular and phenotypic assessment was made of all genotypes Key There was a null phenotypic response to perturbation of Arath;CDC25 expression under control conditions. However, compared with wild type, the arath;cdc25 T-DNA insertion lines were hypersensitive to HU, whereas the Arath;CDC25 over-expressing lines were relatively insensitive. In particular, the over-expressing ...Continue Reading

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Citations

Nov 13, 2015·Annals of Botany·Radka SlovakWolfgang Busch
Jul 6, 2014·Current Opinion in Plant Biology·G Eric SchallerJoseph J Kieber
Oct 13, 2011·The New Phytologist·Wanda M WaterworthChristopher E West
Jan 1, 2013·Biology·Kaoru Okamoto YoshiyamaSeisuke Kimura
May 12, 2011·Annals of Botany·Dennis Francis
May 6, 2019·Journal of Experimental Botany·Benjamin SellesJérémy Couturier
Jun 30, 2011·Annals of Botany·J A Bryant, D Francis
Mar 11, 2021·Annual Review of Plant Biology·Akie ShimotohnoMasaaki Umeda

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