PMID: 8597596Mar 1, 1996Paper

Arabidopsis thaliana cDNA isolated by functional complementation shows homology to serine/threonine protein kinases

Biochimica Et Biophysica Acta
L Covic, R R Lew

Abstract

The yeast Saccharomyces cerevisiae ste6 mutant is defective in transport of a-mating factor, resulting in an inability of ste6 a cells to mate with alpha cells. The gene encodes an ATP-binding cassette, ABC transporter. We used functional complementation of a yeast ste6 mutant with an Arabidopsis thaliana expression library in an attempt to clone an Arabidopsis homolog. Sequence analysis of the isolated Arabidopsis complementing cDNA however showed no homology to the STE6 gene. High sequence similarity was detected to members of the mitogen-activated serine/threonine protein (MAP) kinase family involved in signal transduction: STE20, STE11, BCK1, Byr2 and p65PAK. The Arabidopsis clone failed to complement a fus3/kss1 mutant of S. cerevisiae, but did complement a defect in ste11, ste20, as well as ste6. The isolated clone encodes a protein that is truncated at its amino-terminal, and might function in a similar way as a dominant STE11 truncation allele. These results suggest that the Arabidopsis cDNA encodes a putative serine/threonine kinase that can function in the mating response pathway upstream of FUS3/KSS1 in S. cerevisiae, at the level of STE11 gene. Interestingly, this clone is able to restore the ability of the ste6 yea...Continue Reading

References

Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J ElledgeR W Davis
Jan 1, 1988·Annual Review of Physiology·R S FullerJ Thorner
Jun 1, 1994·Trends in Biochemical Sciences·K J Blumer, G L Johnson
Dec 28, 1993·FEBS Letters·T MizoguchiK Shinozaki
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S W Ramer, R W Davis

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Citations

Jan 1, 2002·The Arabidopsis Book·Liming Xiong, Jian-Kang Zhu
Jul 17, 2001·Physiologia Plantarum·Liming Xiong, Jian-Kang Zhu
Apr 5, 2000·Biochimica Et Biophysica Acta·F L Theodoulou

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