Arabidopsis thaliana GH3.15 acyl acid amido synthetase has a highly specific substrate preference for the auxin precursor indole-3-butyric acid

The Journal of Biological Chemistry
Ashley M SherpJoseph M Jez

Abstract

Various phytohormones control plant growth and development and mediate biotic and abiotic stress responses. Gretchen Hagen 3 (GH3) acyl acid amido synthetases are plant enzymes that typically conjugate amino acids to indole-3-acetic acid (IAA) or jasmonic acid (JA) to inactivate or activate these phytohormones, respectively; however, the physiological and biological roles of many of these enzymes remain unclear. Using a biochemical approach, we found that the Arabidopsis thaliana GH3.15 (AtGH3.15) preferentially uses indole-3-butyric acid (IBA) and glutamine as substrates. The X-ray crystal structure of the AtGH3.15·AMP complex, modeling of IBA in the active site, and biochemical analysis of site-directed mutants provide insight on active site features that lead to AtGH3.15's preference for IBA. Assay-based in planta analysis of AtGH3.15-overexpressing lines indicated that their root elongation and lateral root density were resistant to IBA treatment but not to treatment with either IAA or JA. These findings suggest that AtGH3.15 may play a role in auxin homeostasis by modulating the levels of IBA for peroxisomal conversion to IAA. Analysis of AtGH3.15 promoter-driven yellow fluorescent protein reporter lines revealed that AtGH...Continue Reading

References

Mar 30, 2002·The Journal of Biological Chemistry·Sherry LeClereBonnie Bartel
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Tomoyuki TakaseMinami Matsui
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 8, 2005·Annals of Botany·Andrew W Woodward, Bonnie Bartel
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Feb 6, 2007·The Journal of Biological Chemistry·Jung-Eun ParkChung-Mo Park
Feb 5, 2009·The Journal of Biological Chemistry·Rachel A OkrentMary C Wildermuth
Apr 8, 2009·Nature Chemical Biology·Sandra FonsecaRoberto Solano
Apr 21, 2009·Nature Chemical Biology·Aaron SantnerMark Estelle
Jun 16, 2009·Phytochemistry·Andrzej Bajguz, Alicja Piotrowska
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Jul 20, 2010·The Journal of Biological Chemistry·Qingfeng ChenJoseph M Jez
Dec 15, 2010·Plant Signaling & Behavior·Corey S WestfallJoseph M Jez
Feb 11, 2011·Journal of Experimental Botany·Jutta Ludwig-Müller
Mar 2, 2011·Molecular Plant·Lucia C Strader, Bonnie Bartel
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Sibu Simon, Jan Petrášek
Apr 13, 2013·Journal of Experimental Botany·David A KorasickLucia C Strader
May 28, 2013·The Journal of Biological Chemistry·Corey S WestfallJoseph M Jez
Jul 4, 2013·Sub-cellular Biochemistry·Gretchen M Spiess, Bethany K Zolman
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Adam RoundChloe Zubieta
Feb 11, 2015·American Journal of Botany·Tara A Enders, Lucia C Strader
Apr 18, 2015·BMC Research Notes·Marta MichniewiczLucia C Strader
Jun 17, 2015·Plant & Cell Physiology·Satoko SugawaraHiroyuki Kasahara
Jun 2, 2016·Nature Plants·Zuyu ZhengJoanne Chory
Nov 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Corey S WestfallJoseph M Jez

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Citations

Oct 14, 2018·The Journal of Biological Chemistry·Ashley M SherpJoseph M Jez
Jul 15, 2020·Plant Signaling & Behavior·Sourabh Sengupta, Ronita Nag Chaudhuri
Jul 25, 2019·Frontiers in Plant Science·Suresh Damodaran, Lucia C Strader
Nov 27, 2019·Chemical Communications : Chem Comm·Jing-Yi WengMin-Juan Xu
Jul 23, 2020·Plant & Cell Physiology·Shutaro KanekoHiroyuki Kasahara

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