Arabidopsis thaliana PGR7 encodes a conserved chloroplast protein that is necessary for efficient photosynthetic electron transport.

PloS One
Hou-Sung JungK K Niyogi

Abstract

A significant fraction of a plant's nuclear genome encodes chloroplast-targeted proteins, many of which are devoted to the assembly and function of the photosynthetic apparatus. Using digital video imaging of chlorophyll fluorescence, we isolated proton gradient regulation 7 (pgr7) as an Arabidopsis thaliana mutant with low nonphotochemical quenching of chlorophyll fluorescence (NPQ). In pgr7, the xanthophyll cycle and the PSBS gene product, previously identified NPQ factors, were still functional, but the efficiency of photosynthetic electron transport was lower than in the wild type. The pgr7 mutant was also smaller in size and had lower chlorophyll content than the wild type in optimal growth conditions. Positional cloning located the pgr7 mutation in the At3g21200 (PGR7) gene, which was predicted to encode a chloroplast protein of unknown function. Chloroplast targeting of PGR7 was confirmed by transient expression of a GFP fusion protein and by stable expression and subcellular localization of an epitope-tagged version of PGR7. Bioinformatic analyses revealed that the PGR7 protein has two domains that are conserved in plants, algae, and bacteria, and the N-terminal domain is predicted to bind a cofactor such as FMN. Thus, ...Continue Reading

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Citations

Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aiguo ZhaoLin Liu
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Sarah S Conte, Alan M Lloyd
Dec 18, 2013·Plant, Cell & Environment·Michael ScharfenbergTatjana Kleine
Jan 10, 2013·Plant Signaling & Behavior·Olaf Czarnecki, Bernhard Grimm

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Datasets Mentioned

BETA
AY087867
BT002574

Methods Mentioned

BETA
X-ray
PCR
fluorescence microscopy

Software Mentioned

SPSS Graduate Pack
dCAPS Finder
BLASTP
MAFFT
Excel
hmmpfam
uniqueseq
IP lab
MUSCLE
UCSC SAM

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