Arabidopsis thaliana proliferating cell nuclear antigen has several potential sumoylation sites

Journal of Experimental Botany
Wojciech StrzalkaHalina Gabrys

Abstract

Proliferating cell nuclear antigen (PCNA) is post-translationally modified in yeast and animal cells. Major studies carried out in the last decade have focused on the role of sumoylated and ubiquitinated PCNA. Using different approaches, an interaction between plant PCNA and SUMO both in vivo and in bacteria has been demonstrated for the first time. In addition, identical sumoylation patterns for both AtPCNA1 and 2 were observed in bacteria. The plant PCNA sumoylation pattern has been shown to differ significantly from that of Saccharomyces cerevisiae. This result contrasts with a common opinion based on previous structural analysis of yeast, human, and plant PCNAs, which treats PCNA as a highly conserved protein even between species. Analyses of AtPCNA post-translational modifications using different SUMO proteins (SUMO1, 2, 3, and 5) revealed similar modification patterns for each tested SUMO protein. Potential target lysine residues that might be sumoylated in vivo were identified on the basis of in bacteria AtPCNA mutational analyses. Taken together, these results clearly show that plant PCNA is post-translationally modified in bacteria and may be sumoylated in a plant cell at various sites. These data open up important new...Continue Reading

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Citations

Jan 15, 2013·Journal of Experimental Botany·Wojciech StrzalkaKatsunori Tanaka
Nov 12, 2013·Journal of Experimental Botany·Juan C Del Pozo, Concepción Manzano
Sep 18, 2015·Frontiers in Plant Science·Wojciech K StrzalkaAgnieszka K Banas
Jul 30, 2020·The Biochemical Journal·Li FanWei Xiao
Nov 13, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jesus S Carrasco-MirandaLuis G Brieba
Dec 6, 2020·Genes·Wojciech StrzałkaAgnieszka Katarzyna Banaś
Feb 18, 2021·Plant & Cell Physiology·Justyna ŁabuzWojciech Strzałka

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