Arabidopsis Trichome Contains Two Plasma Membrane Domains with Different Lipid Compositions Which Attract Distinct EXO70 Subunits

International Journal of Molecular Sciences
Zdeňka KubátováIvan Kulich

Abstract

Plasma membrane (PM) lipid composition and domain organization are modulated by polarized exocytosis. Conversely, targeting of secretory vesicles at specific domains in the PM is carried out by exocyst complexes, which contain EXO70 subunits that play a significant role in the final recognition of the target membrane. As we have shown previously, a mature Arabidopsis trichome contains a basal domain with a thin cell wall and an apical domain with a thick secondary cell wall, which is developed in an EXO70H4-dependent manner. These domains are separated by a cell wall structure named the Ortmannian ring. Using phospholipid markers, we demonstrate that there are two distinct PM domains corresponding to these cell wall domains. The apical domain is enriched in phosphatidic acid (PA) and phosphatidylserine, with an undetectable amount of phosphatidylinositol 4,5-bisphosphate (PIP2), whereas the basal domain is PIP2-rich. While the apical domain recruits EXO70H4, the basal domain recruits EXO70A1, which corresponds to the lipid-binding capacities of these two paralogs. Loss of EXO70H4 results in a loss of the Ortmannian ring border and decreased apical PA accumulation, which causes the PA and PIP2 domains to merge together. Using tr...Continue Reading

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Citations

Feb 18, 2020·The Plant Journal : for Cell and Molecular Biology·Přemysl PejcharMartin Potocký
May 23, 2020·Annual Review of Plant Biology·Lise C Noack, Yvon Jaillais
Apr 25, 2020·International Journal of Molecular Sciences·Elisabeth Jamet, Christophe Dunand
Feb 2, 2021·Frontiers in Plant Science·Antonietta SaccomannoViktor Žárský
Sep 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lukáš SynekMartin Potocký

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence recovery after photobleaching
transmission electron microscopy
light microscopy
FCS
Transgenic

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